30例复发性外阴阴道念珠菌病患者致病菌株分析  

Analysis of pathogenic strains isolated from 30 cases of RVVC

作　　者：高飞[1] 曹萍[1] 吴颖[1] 董跃兰[2] 霍克克[3] 王亚倩[3] 

机构地区：[1]云南省第一人民医院皮肤科,云南昆明650032 [2]云南省第一人民医院妇科,云南昆明650032 [3]复旦大学生命科学院,上海200433

出　　处：《重庆医学》2013年第13期1466-1467,1470,共3页Chongqing medicine

基　　金：云南省科技厅应用基础计划资助项目(2009ZC161M)

摘　　要：目的探讨复发性外阴阴道念珠菌病(RVVC)患者阴道和肛管念珠菌的异同。方法收集30例RVVC患者阴道和肛管的菌株,三色荧光分别标记保守基因CDC3、EF3和HIS3微卫星序列引物,PCR扩增,产物行聚丙烯酰胺凝胶电泳。GeneScan软件扫描获取片段精确长度,Genotype软件基因分型。结果 30例患者中,有17例患者阴道和肛管的菌株为白色念珠菌,且基因型彼此相同。结论 RVVC患者阴道的菌株可能从肛管移行而来,这可能是导致该病反复发作的重要因素之一。Objective To compare the genotypes of candida strains isolated from the vagina and anal tube in the patients with recurrent vulvovaginal candidiasis（RVVC）.Methods Candida stains isolated from the vagina and anal tube in 30 cases of RVVC were collected.The 3-color fluorencence was adopted to label the conservative gene CDC3,EF3 and HIS3 microsatellite sequence primers for amplification by PCR.Then,the PCR products were performed the electrophoresis by polyacrylamide gel,the sizes of products were read with GeneScan software and followed by genotyping with Genotyper software.Results Among 30 cases,17 strains in vagina and anal tube were candida albicans with same genotype each other.Conclusion For the patient with RVVC,the bacterial strains can migrate from anal tube to vagina,which might be one of important factors causing disease recurrence.

关 键 词：念珠菌病 外阴阴道 基因型 微卫星 

分 类 号：R711.3[医药卫生—妇产科学]