颈交感神经阻滞对烧伤大鼠蓝斑放电的影响  

Effect of cervical sympathetic block on electric discharge of locus ceruleus neurons in burned rats

作　　者：郭晓姝[1] 巩继平[2] 尚改萍[1] 张翠英[1] 

机构地区：[1]长治医学院生理教研室,山西长治046000 [2]新乡市中心医院麻醉科,河南新乡453000

出　　处：《重庆医学》2013年第14期1598-1600,1603,共4页Chongqing medicine

基　　金：山西省高等学校科技研究开发自选项目(20091133)

摘　　要：目的探讨颈交感神经阻滞(SB)对烧伤大鼠蓝斑神经元电生理变化的影响。方法成年健康雄性Wistar大鼠20只,随机分为4组,对照组、对照SB组、烧伤组和烧伤SB组,每组5只。总体表面积(total body surface area,TBSA)20%Ⅲ度大鼠烧伤模型,4h后观察蓝斑神经元放电,HE染色组织学定位,免疫组织化学方法验证。结果在放电频率、放电脉冲间隔(ISI)、众数和不对称指数(AI),对照组和对照SB组比较差异无统计学意义(P>0.05);烧伤组和烧伤SB组放电频率显著高于对照组和对照SB组(P<0.01),而烧伤组又显著高于烧伤SB组(P<0.01);烧伤组和烧伤SB组ISI和众数显著高于对照组和对照SB组(P<0.01),而烧伤SB组又显著高于烧伤组(P<0.01);烧伤组和烧伤SB组AI显著高于对照组和对照SB组(P<0.01),而烧伤SB组和烧伤组间差异无统计学意义(P>0.05)。免疫组织化学方法显示蓝斑神经元主要为多巴胺β羟化酶(DβH)神经元。结论 SB对烧伤的治疗作用中枢机制之一可能是通过抑制蓝斑神经元放电活动来实现。Objective To investigate the effect of cervical sympathetic block（SB） on the electrophysiology change of locus ceruleus neurons in burned rats.Methods 20 healthy,male adult Wistar rats were divided into 4 groups randomly and equally,control group,control＋SB group,burned group and burned ＋ SB group,5 cases in each group,with burned injury of TBSA 20% Ⅲ°.The discharage style of locus ceruleus neurons were observed after 4 h.The location of locus ceruleus was confirmed by HE staining and validated by immunohistochemical method.Results The mean discharge frequency,mean ISI,mean mode and asymmetry index of hypothalamus neurons had no statistical difference between the control group and the control＋SB group（P0.05）;the mean discharge frequency of neurons in the burned group and the burned＋SB group were significantly higher than that in the control group and the control＋SB group（P0.01）,and burned group were significantly higher than the burned＋SB group（P0.01）.The mean ISI and mean mode of neurons in the burned group and the burned＋SB group were significantly higher than those in the control group and the control＋SB group（P0.01）,and burned＋SB group were significantly higher than burned group（P0.01）.The asymmetry index（AI） of neurons in the burned group and the burned＋SB group were significantly higher than that in the control group and the control＋SB group（P0.01）,and difference between the burned group and the burned ＋ SB group had no statistical significance（P0.05）.The immunohistochemical method showed that locus ceruleus neurons were mainly DβH neurons.Conclusion One of central mechanisms of therapeutical effect of SB to burned injury may be to inhibit the electric discharge activity of locus ceruleus neurons.

关 键 词：烧伤 自主神经传导阻滞 蓝斑 放电形式 

分 类 号：R644[医药卫生—外科学]