基于RAPD分子标记与表型标记的柴松分类地位研究  

Analysis of the Taxonomical Position of Pinus tabulaeformis.f.Shekannesis Based on RAPD Markers and Morphological Traits

作　　者：解庆[1] 刘志红[1] 李周岐[1] 原忠林 王忠钰 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100 [2]辽宁省鞍山市千山风景区管委会林业局,辽宁鞍山114045

出　　处：《林业科学研究》2013年第2期151-155,共5页Forest Research

基　　金：国家自然科学基金"柴松分类地位及其遗传多样性研究"(30972382)

摘　　要：采用RAPD分子标记方法与表型标记的方法对柴松的分类地位进行了研究,结果表明:20条随机引物共扩增出176条条带,其中多态性带为172条,多态性百分率为97.73%。同时对24个形态性状进行了统计分析。对RAPD试验结果和形态统计的结果分别采用UPGMA法聚类分析,聚类的结果类似,认为柴松尚未达到油松变种或近缘种一级的分类水平。In this study, the taxonomical position of Pinus tabulaeformis f. Shekannesis Yao et Hsu was estimated on the basis of morphological characters and RAPD data. For RAPD analysis, 20 random primers were selected and 176 bands, within 172 polymorphic bands and a polymorphism rate of 97.73%, were obtained. Twenty four mor-phological characters data were also recorded. The RAPD and morphological characters data were then respectively analyzed with an unweighted pair-group method using an arithmetic average （UPGMA）. The results based on differ-ent data are similar and it indicates that the Pinus tabulaeformisf Shekannesis Yao et Hsü has not yet reached a vari-ant or relatives of Pinus tabulaeformis.

关 键 词：柴松 RAPD 表形标记 分类地位 

分 类 号：S718.46[农业科学—林学]