毛细管电泳紫外检测法同时测定白术中白术内酯Ⅱ和白术内酯Ⅲ含量  

Simultaneous Determination of Butenolide Ⅱ and Ⅲ in Atractylodes Macrocephala Koidz by Capillary Electrophoresis with Ultraviolet Detection

作　　者：石冬琴[1,2] 王荣[2] 田薇[1] 谢华[2] 

机构地区：[1]浙江农林大学林业与生物技术学院,浙江临安311300 [2]兰州军区兰州总医院临床药理基地,兰州730050

出　　处：《解放军医药杂志》2013年第4期65-67,70,共4页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：全军"十二五"面上项目(BWS2012JO12)

摘　　要：目的建立同时测定白术中白术内酯Ⅱ、Ⅲ的毛细管电泳紫外检测方法,并测定白术样品中白术内酯Ⅱ、Ⅲ的含量。方法采用毛细管区带电泳法,紫外检测波长254 nm,缓冲液硼酸-硼砂体积比为5∶1,分离电压15 kV,柱温20℃,电动进样10 s。结果在最优条件下,白术内酯Ⅱ、Ⅲ完全分离;在线性范围内与峰面积线性关系良好,白术内酯Ⅱ线性方程为Y=2724.5X-54.078(r=0.9958),白术内酯Ⅲ的线性方程为Y=244.65X+201.8(r=0.9995),检测限(S/N=3)为0.2和1.0μg/ml。结论毛细管电泳紫外检测法可用于白术中白术内酯Ⅱ、Ⅲ的含量测定。Objective To establish a capillary electrophoresis with ultraviolet detection （CE-UV） method for simultaneous determination of butenolide Ⅱ and Ⅲ in atractylodes macrocephala, and to detect contents of butenolide Ⅱ and Ⅲ in atractylodes macrocephala koidz. Methods The method was used by capillary zone eleetrophoresis, whose system included a 254 nm ultraviolet wavelength, the 5 ： 1 volume ratio of boric acid-borax buffer solution, 15 kV running voltage, 15℃ of column temperature and the 10 s of electrokinetic injection. Results In the optimization condition, butenolide Ⅱ was completely separated from butenolide Ⅲ; and in linear range with peak area had good liner relationship, linear equation of butenolide Ⅱ was Y = 2724.5X - 54. 078 （ r = 0. 9958） , and linear equation of butenolide m was Y =244.65×＋201.8 （r =0. 9995） , two detection limits （S/N =3） for butenolide Ⅱ and Ⅲ were 0.2 μg/ml and 0. 1 μg/ml respectively. Conclusion The capillary electrophoresis with ultraviolet detection method may apply to quantitate butenolide Ⅱ and Ⅲ in atractylodes macrocephala koidz.

关 键 词：白术内酯Ⅱ 白术内酯Ⅲ 白术 电泳 毛细管 分光光度法 紫外线 

分 类 号：R931.5[医药卫生—生药学]