高效液相色谱法测定清咽六味散中槲皮素的含量  

Determination the Content of Quercetin in Qingyanliuwei San by HPLC Method

作　　者：金丽杰[1] 

机构地区：[1]内蒙古民族大学化学化工学院,内蒙古通辽028043

出　　处：《内蒙古民族大学学报（自然科学版）》2013年第1期77-80,共4页Journal of Inner Mongolia Minzu University：Natural Sciences

摘　　要：目的:建立高效液相色谱法测定清咽六味散中槲皮素含量的方法.方法:采用Hypersil ODS-C18(250mm×4.6mm,5um)色谱柱,流动相为甲醇-水(70:30),检测波长360nm,流速0.80ml/min,柱温为30℃.结果:槲皮素质量与峰面积有良好的线性关系,线性范围为0.02～0.4μg(r=0.9997).槲皮素的平均加样回收率为98.90%,RSD=1.29.结论:该方法简单、重现性好,结果准确可靠,可用于清咽六味散中槲皮素的质量控制.Objective： To establish an HPLC method for determination the Quercetin in Qingyanliuwei San. Methods： Using a Hypersil ODS-C18 （250mm×4.6mm,5um）, mobile phase was methanol-water （70：30）, detection wavelength was $60nm, flow velocity was 0.800ml/min, column temperature was 30℃. Results： Quercetin quantity and the peak area has a good linear relationship between the linear range of 0.02- 0.4ug （r=0.9997）. The average recovery rate was 98.90%, RSD=1.29. Conclusion： The method is simple, reliable, reproducible, accurate, and can be used as quality control of quercetin in Qingyanliuwei San.

关 键 词：高效液相色谱法 清咽六味散 槲皮素 

分 类 号：R927.2[医药卫生—药学]