槟榔有效组分的提取分离及其对大鼠胃平滑肌收缩作用影响的研究  

Study on Extraction Separation of Areca Catechu and the Effect on Gastric Smooth Muscle Contraction in Rats

作　　者：李晨[1] 范尧夫[2] 吕涛[2] 魏睦新[2] 胡兵[1] 

机构地区：[1]南京中医药大学附属徐州市中医院,江苏徐州221000 [2]南京医科大学第一附属医院,江苏南京210029

出　　处：《中医学报》2013年第5期683-685,共3页Acta Chinese Medicine

基　　金：江苏省自然科学基金课题(编号:SBK200920943);江苏省中医药管理局课题(编号:LZ09108)

摘　　要：目的:探讨槟榔的有效组分F57对大鼠胃平滑肌收缩运动的影响,并初步分析其作用机制。方法:建立高效液相色谱法从槟榔中分离有效组分F57的方法,采用膜过滤和凝胶排阻色谱法(Sephadex G-25)对槟榔成分进行分离纯化,观察不同浓度F57对大鼠胃平滑肌的收缩作用;再分别予以阻断剂酚妥拉明、阿托品和维拉帕米孵育肌条,观察F57对肌条的收缩效应。结果:①从槟榔中分离出有效组分F57,其含有的槟榔碱在18.6～112.7μg范围内呈线性关系(r=0.999 7);②不同浓度F57均可增加大鼠离体胃平滑肌肌条的收缩力和收缩振幅,与对照组比较有显著增高(P<0.01);③阻断剂维拉帕米和阿托品对F57引起的肌条收缩有抑制作用(P<0.05)。结论:高效液相色谱法对F57的分离准确、简便易行、重复性好;F57对大鼠离体胃平滑肌条的收缩有明显兴奋作用,其引起的收缩效应可能与钙通道、M-胆碱能受体有关。Objective ： To discuss F57 of areca catechu on gastric smooth muscle contraction in rats, and analyse the mechanism. Meth- ods ： HPLC was adopted to separate 1757 from areca catechu. Membrane fihration and exclusion chromatography were used for separation and purification of areca catechu. Observed the effect of different concentration 1757 on gastric smooth muscle contraction. Then phento- lamine, atropine and verapamil were treated with muscel, and observed contraction by F57. Resuhs：①F57 that was separated from areca catechu containing arecoline. Arecoline showed a good linear relationship in range of 18.6 - 112.7μg（ r = 0. 9997 ）. ②Compared with control group, different concentration 1757 increased contractility and contraction amplitude in gastric smooth muscle. There were signifi- cant differently（P 〈0.01 ）. ③Verapamil and atropine inhibited smooth muscle contraction caused by F57 （P 〈 0.05 ）. Conclusions： HPLC has accuracy and good reproducibility. F57 can obviously restrain smooth muscle contraction in rats. The mechanism may associ- ate with calcium channel and M-cholinergic receptor.

关 键 词：槟榔 大鼠 平滑肌 高效液相色谱法 阻断剂 

分 类 号：R285.5[医药卫生—中药学]