东北设施黑土土壤微生物总DNA提取方法探讨  

Microbial DNA Extraction Method about Facilities Black Soil in Northeast China

作　　者：刘昕昕[1] 孟令波[2] 潘明阳[1] 李淑敏[1] 

机构地区：[1]东北农业大学资源与环境学院,黑龙江哈尔滨150030 [2]哈尔滨学院理学院,黑龙江哈尔滨150086

出　　处：《生物技术》2013年第2期45-48,共4页Biotechnology

基　　金：国家自然科学基金项目(31071867);第二批中国博士后科学基金特别资助(200902367);黑龙江省博士后科研启动资金项目(LBH-Q10146)资助

摘　　要：目的:找到一种适合东北设施高腐殖含量黑土土壤微生物总DNA简单易行的提取方法。方法:采用5种不同的DNA提取方法进行土壤总DNA提取。结果:5种提取方法的DNA产量在6.88～29.71μg/g,OD260/OD280值为1.03～1.27,OD260/OD230值为0.65～0.88,经纯化后均可进行PCR扩增。但经改进的方法 E提取DNA产量最高,纯度最好。结论:方法 E—加入TENP和PBS缓冲液预处理的提取方法是一种适宜东北设施黑土土壤微生物总DNA批量提取简单易行的方法。Objective：To seek out a simple extraction method for total DNA of soil microorganism for high humus content northeast facilities black soil. Method：Five different DNA extracting methods were adopted to process soil total DNA extraction. Result： The DNA yield of five extraction methods were in the range of 6. 88 -29.71 p.g/g. The ratio of OD260/OD280 was in the range of 1.03 - 1.27, and the ratio of OD260/OD230 was in the range of 0.65 -0. 88, which could process PCR amplification after purification. But DNA of highest yield and best purity was extracted by the improved method E. Conclusion：The method E, within the buffer solution TENP and PBS pretreatment, is a simple method for total DNA batch extraction of soil microorganism for northeast black soil.

关 键 词：东北设施黑土 土壤微生物 DNA提取 PCR 

分 类 号：Q751[生物学—分子生物学]