外源DNA导入大豆变异后代的SOD同工酶分析  

STUDY ON THE SUPEROXIDE DISMUTASE (SOD) FROM THE VARIANTS INDUCED BY EXOGENOUS DNA INTRODUCTION INTO SOYBEAN (GLYCINE MAX. )

作　　者：刘德璞[1] 袁鹰[1] 庄炳昌[1] 

机构地区：[1]吉林省农业科学院大豆研究所

出　　处：《大豆科学》1991年第3期194-199,共6页Soybean Science

摘　　要：以花粉管通道途径向大豆导入外源DNA,变异后代已达D_4代。 受体为栽培品种吉林20号、吉林16号;供体足野生豆、半野生豆和栽培豆。从439个D_1植株中得12株变异株,变异率为3％。变异性状明显表现供体特征,并且是可遗传的。超氧物歧化酶(SOD)同工酶电泳鉴定,在有的变异株中检测到供体具有的亚基谱带b_1、b_2。结果表明,外源DNA导入技术在大豆育种上是可以利用的。DNA of the wild, semi-wild and cultivarted soybean was introduced into soybean cultivars Jilin 20, Jilin16, through pollen tube pathway after soybean self-fertilizing. The results of su peroxide dismutase (SOD) electrophoresis showed that some receptors and their offsprings increased two bands b1, b2, which were present in the donors but were absent in receptors. These variations were observed in offsprings from D1 to D4 generations. The results showed that exoge nous DNA segments can insert into receptor's soybean genome and express. This techninque may be used for soybean breeding.

关 键 词：大豆 外源DNA 后代 SOD 同功酶 

分 类 号：S565.103.2[农业科学—作物学]