辽宁省2009年至2011年病毒性腹泻流行病学研究  

Epidemiological study on viral diarrhea during 2009--2011 in Liaoning Province

作　　者：安淑一[1] 赵卓[2] 郭军巧[2] 韩悦[3] 王作虪[1] 任毅[3] 周宝森[1] 

机构地区：[1]中国医科大学流行病与卫生统计教研室,沈阳110001 [2]辽宁省疾病预防控制中心主任办公室 [3]辽宁省疾病预防控制中心免疫规划所

出　　处：《中华传染病杂志》2013年第3期166-169,共4页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金资助项目（30771860）

摘　　要：目的通过监测辽宁省哨点医院病毒性腹泻病例，了解病毒性腹泻病原流行特征，探讨病毒性腹泻病原变异和流行规律。方法收集2009年1月至2011年12月辽宁省哨点医院粪便标本639份，采用PCR和RT-PCR检测轮状病毒、杯状病毒、星状病毒和腺病毒。计数资料分析采用7。检验或Fisher确切概率法。结果639份粪便标本中轮状病毒阳性率为15．96％，杯状病毒为11．25％，星状病毒为1．25％，腺病毒为0．31％。检出A组轮状病毒101份，G3、P[8]型为优势株；C组轮状病毒1份，为辽宁省首次检出，并通过分子生物学分析，c组轮状病毒核苷酸序列JX407109与日本暴发疫情核苷酸序列AB648916亲缘关系较近，核苷酸序列一致性达到99％。检出杯状病毒72份，包括诺如病毒70份，为GⅡ／4型，札如病毒2份。检出星状病毒8份，以1型为主。检出腺病毒2份为41型。辽宁省病毒性腹泻高发月份为12月至次年2月，轮状病毒和星状病毒以5岁以下儿童高发，杯状病毒感染无年龄差异。结论辽宁省病毒性腹泻主要病原为A组轮状病毒和杯状病毒，c组轮状病毒与日本暴发疫情核苷酸序列较近，需引起注意。Objective To understand the epidemiologic characteristics and pathogenic virus of cases of viral diarrhea in sentinel hospitals in Liaoning Province. Methods From Jan 2009 to Dec 2011, 639 stool samples from sentinel hospitals of Liaoning Province were collected. Rotavirus, human calicivirus, astrovirus and adenovirus were detected by polymerase chain reaction and reverse transcriptase-polymerase chain reaction. The data analysis used chi-squanetest and Fisher^s exact test. Results Rotavirus, human calicivirus, astrovirus and adenovirus were detected in 15.96 ~, 11.25 ~, 1.250~ and 0.31 ~ of the 639 specimens, respectively. G3 was the most prevailing serotype and P[8] was the most common genotype among 101 group A rotavirus isolates. One strain of group C rotavirus was also detected, which was reported for the first time from Liaoning Province. Phylogenetic analysis showed that this group C rotavirus JX407109 in the present study had the closest genetic relationship with the outbreak strain AB648916 from Japan, with nucleotide sequence consistency of 99 ~. Among the 72 samples of human calicivirus, 70 samples were norovirus with G l]/4 being the predominant genotype, and 2 samples were sapovirus. Astrovirus was detected in 8 samples, most of which were genotype 1. Adenovirus was detected in 2 samples, and both were genotype 41. High incidences ofviral diarrhea were noted during the months from December to next year February, and children under 5 years of age had high incidence of rotavirus and astrovirus, while the incidence of calicivirus were similar among different age groups. Conclusions The predominant pathogens of viral diarrhea in Liaoning Province are group A rotavirus and calicivirus. Notably, the group C rotavirus in Liaoning Province shares high genetic consistency with the outbreak strain from Japan.

关 键 词：腹泻 流行病学研究 基因型 轮状病毒属 碱基序列 

分 类 号：R181[医药卫生—流行病学] R51[医药卫生—公共卫生与预防医学]