中药五倍子提取物对牙本质表面性能的强化作用  

Effect of Galla chinensis on the surface strengthening of bovine dentine in vitro

作　　者：邓盟[1] 黎红[2] 董孝立[1] 施桔红[2] 

机构地区：[1]口腔疾病研究国家重点实验室,四川大学,四川成都610041 [2]浙江中医药大学口腔医学院,杭州口腔医院,浙江杭州310006

出　　处：《上海口腔医学》2013年第2期164-168,共5页Shanghai Journal of Stomatology

基　　金：四川省科技支撑项目(2009FZ0065);浙江中医药大学校基金(2012)~~

摘　　要：目的:评价中药五倍子对牙本质抗酸作用及表面强化的影响。方法:45个牛牙本质标本随机分为3组,以4 g/L五倍子提取液为实验药物,1 g/L NaF为阳性对照组,去离子水为阴性对照组,同时进行体外pH循环致龋实验。采用显微硬度仪测定各样本pH循环前后表面显微硬度,观察牙本质显微硬度的变化,并用扫描电镜和X线能谱仪分析pH循环后各组牙本质表面形貌和元素含量。数据采用SPSS17.0软件包进行统计学分析。结果:与去离子水相比,4 g/L五倍子可以显著减少牙本质表面显微硬度的降低(P<0.05),效果与1 g/L NaF无显著差异(P>0.05)。五倍子组和NaF组牙本质小管大部分呈闭合和堵塞状,而去离子水组牙本质小管呈开放状。五倍子组和NaF组的Ca和P成分显著高于去离子水组(P<0.05)。结论:五倍子可增强牙本质表面的抗酸能力,强化牙本质的表面性能,有抑制牙本质龋进展、保护牙本质免受酸腐蚀的作用。PURPOSE： To evaluate the effect of Galla chinensis extract （GCE） on strengthening the surface properties of bovine dentine in vitro. METHODS： Forty-five bovine dentine blocks were prepared and randomly divided into three groups to undergo a modified anti-acid pH-cycling regimen. Treatments were 4 g/L aqueous solution of Galla chinensis, 1 g/L NaF （positive control） and distilled deionized water （DDW, negative control）. Surface dentine microhardness was assessed before and after the pH-cycling regimen and the percentage of microhardness loss （PML） was calculated. Then specimens were analyzed with scanning electron microscopy （SEM） and energy-dispersive X-ray spectrometry （EDXS）. Statistical analysis was performed by SPSS17.0 software package. RESULTS： Compared with DDW, GCE significantly reduced dentinal microhardness loss （P〈0.05）. No significant difference of the PML was found between GCE and NaF groups （P 〉0.05）. Both SEM images and elemental contents were similar between the GCE and NaF group. CONCLUSIONS： Galla chinensis can enhance the surface properties of bovine dentine and may inhibit dentine caries progression and protect tooth dentine from erosion in this laboratory study. Supported by Science and Technology Support Program of Sichuan Province （2009FZ0065） and Research Fund of Zhejiang Chinese Medicine University （2012）.

关 键 词：五倍子 牙本质龋 抗酸性 显微硬度 表面强化 酸蚀 

分 类 号：R285[医药卫生—中药学]