格列美脲对高糖环境下大鼠下颌骨成骨细胞增殖、分化及矿化的影响  

Effects of glimepiride on proliferation,differentiation and mineralization of rat mandibular osteoblasts in hyperglycemia

作　　者：马攀[1] 谭包生[1] 刘洪臣[2] 顾斌[2] 马军利[2] 鄂玲玲[2] 吴霞[2] 

机构地区：[1]首都医科大学附属北京口腔医院种植中心,北京100050 [2]解放军总医院口腔科.北京100853

出　　处：《上海口腔医学》2013年第2期175-180,共6页Shanghai Journal of Stomatology

摘　　要：目的:探讨格列美脲对高糖培养的大鼠下颌骨成骨细胞增殖、分化和矿化功能的影响。方法:分离培养大鼠下颌骨成骨细胞,分别给予5.5 mmol/L(生理糖浓度)、16.5 mmol/L葡萄糖浓度的培养液培养,加入或不加入10μmol/L格列美脲后,用噻唑蓝法(MTT)观察成骨细胞7 d时的增殖情况,生化法测定7 d时的碱性磷酸酶(alkaline phosphatase,ALP)活性,Western免疫印迹检测7 d和14d时Ⅰ型胶原(collagenⅠ,ColⅠ)的表达,实时定量PCR检测21 d时的骨钙素(osteocalcin,OCN)mRNA的表达,采用SPSS13.0软件包对数据进行统计学分析。结果:高糖浓度降低了成骨细胞的增殖、ALP活性和OCN的mRNA表达,提高了14 d时的ColⅠ的表达。格列美脲能够促进2种糖浓度下的成骨细胞增殖、ALP活性、ColⅠ的蛋白表达和OCN的mRNA表达。结论:高糖浓度降低了大鼠下颌骨成骨细胞的增殖、分化和矿化能力,在2种糖浓度下,格列美脲促进大鼠下颌骨成骨细胞的增殖、分化和矿化。PURPOSE： To evaluate the effects of hyperglycemia and glimepiride on proliferation, differentiation and mineralization of rat mandibular osteoblasts to verify the hypothesis of dental implant administration. METHODS： Primary osteoblasts were isolated and cultured. Then the cells were placed in an osteogenic medium, containing 2 different concentrations of glucose （5.5 mmol/L and 16.5 mmol/L）, with or without glimepiride （10 p, mol/L）. Cell proliferation was evaluated through MTF assay. Alkaline phosphatase （ALP） activity was determined by biochemistric method. Col I protein levels were determined by Western blot. OCN mRNA levels were tested by RT-PCR. SPSS 13.0 software package was used for statistical analysis. RESULTS： Hyperglycemic conditions interfered with the proliferation, ALP activity and OCN mRNA expression of rat osteoblasts, but improved the expression of Col I on day 14. Glimepiride stimulated rat osteoblast proliferation, ALP activity and OCN mRNA expression. The addition of glimepiride to normoglycemic （5.5 mmol/L） cultures registered a significant increase of Col I expression at 7 d and 14 d. Glimepiride significantly increased Col I expression in cells cultured with 16.5 mmol/L glucose for 7 days, but failed to increase at 14 d. CONCLUSIONS： Hyperglycemic conditions interfered with the proliferation, differentiation and mineralization of osteoblasts in rats; however, glimepiride improved the proliferation, differentiation and mineralization of osteoblasts in rats.

关 键 词：格列美脲 成骨细胞 增殖 分化 

分 类 号：R965[医药卫生—药理学]