夹脊、督脉电针对大鼠脊髓损伤前炎性细胞因子表达的影响  

Impaction of Jiaji and Du Meridian electroacupuncture treatment on expressions of pro-inflammatory cytokines in spinal cord injury of rats

作　　者：王赛[1] 邹恩苗[1] 林海燕[1] 屠文展[1] 李江茹[1] 程博[1] 蒋松鹤[1] 

机构地区：[1]温州医学院附属第二医院康复科,325027

出　　处：《中国康复医学杂志》2013年第5期389-392,共4页Chinese Journal of Rehabilitation Medicine

基　　金：温州市科技局资助项目(Y20110069)

摘　　要：目的:观察夹脊、督脉不同配穴的电针对脊髓损伤(SCI)后的大鼠前炎性细胞因子表达的影响。方法:SD大鼠32只随机分为4组:假模组(n=8)、模型对照组(n=8)、夹脊电针组(n=8)、督脉电针组(n=8)。夹脊电针组、督脉电针组于SCI后0.5h和4h分别在夹脊与督脉的穴位进行1次电针治疗。SCI后8h,每组8只大鼠行Western-blot检测脊髓IL-1β、IL-6、TNF-α蛋白含量的表达。结果:假模组、夹脊电针组、督脉电针组IL-lβ、IL-6、TNF-α的蛋白含量均低于模型对照组,差异有显著性意义(P<0.05)。夹脊电针组、督脉电针组之间差异无显著性意义(P>0.05)。结论:夹脊、督脉电针可以通过抑制SCI后IL-lβ、IL-6、TNF-α3种前炎性细胞因子的表达,抑制SCI急性期的炎症反应来保护脊髓,减轻损伤的发生,起到神经保护作用。Objective： To observe the expressions of pro-inflammatory cytokines of spinal cord injury（SCI） rats undertaking electroacupuncture treatment（EA） at Jiaji and Du Meridian. Method： Thirty-two SD rats were randomly divided into four groups： sham module group（n=8）, control module group （n=8）, Jiaji EA group （n=8）, Du Meridian EA group （n=8）. Jiaji EA group and Du Meridian EA group were treated with EA treatment at points of Jiaji and Du Meridian 0.5h and 4h after SCI, respectively. 8h after SCI, Western-blot was used to detect the expressions of IL-113, IL-6 and TNF-ct protein in SCI section. Result： Compared with control module group, the expressions of IL--1 13, IL-6 and TNF-a proteins SCI sections were significantly lower than those in the sham module group, Jiaji EA group, and Du Meridian EA group（P〈 0.05）. No significant difference was detected between Jiaji EA group and Du Meridian EA group（P〉0.05）. Conclusion： Jiaji and Du Meridian EA could inhibit high expressions of IL-1 13, IL--6 and TNF-ct after SCI, inhibit inflammatory response in acute phase of SCI to protect the spinal cord, reduce the occurrence of injury, to play neuroprotective effect.

关 键 词：脊髓损伤 电针治疗 夹脊 督脉 神经保护 

分 类 号：R246.2[医药卫生—针灸推拿学]