活性短肽修饰纳米晶胶原基骨修复拔牙后剩余牙槽嵴  

Nano-hydroxyapatete/collagen/P17-bone morphogenetic protein-2 for alveolar ridge repair after tooth extraction

作　　者：李善昌[1] 龚立强 杜晓岩[2] 

机构地区：[1]佳木斯大学口腔医学院颌外二科,黑龙江省佳木斯市154007 [2]佳木斯大学口腔医学院整形美容外科,黑龙江省佳木斯市154007

出　　处：《中国组织工程研究》2013年第12期2138-2144,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:各种原因导致的牙齿拔除后都会造成剩余牙槽嵴的吸收,导致颌骨骨量不足,不利于患者后期接受义齿和种植牙的修复治疗。目的:通过建立大鼠拔牙后剩余牙槽嵴模型,观察纳米晶胶原基骨/活性肽P17-骨形态发生蛋白2对拔牙窝修复及牙槽嵴吸收的影响。方法:将36只健康SD大鼠随机分为实验组、对照组和单纯材料组,均拔除右侧下颌中切牙,实验组在拔牙窝内即刻植入纳米晶胶原基骨/活性肽P17-骨形态发生蛋白2材料,对照组在拔牙窝内植入纳米晶胶原基骨/骨形态发生蛋白2材料,单纯材料组在拔牙窝内植入纳米晶胶原基骨材料。结果与结论:①牙槽嵴的相对长度:术后2,4,8周,实验组与对照组均大于单纯材料组(P<0.05),实验组与对照组比较差异无显著性意义(P>0.05)。②组织学观察结果:术后2,4,8周,实验组与对照组新生血管和新骨形成的速度和质量均优于单纯材料组。③新生骨面积占骨缺损面积的百分比:术后2,4,8周,各组随时间增长不断增加,实验组与对照组大于单纯材料组(P<0.05),对照组大于实验组。表明纳米晶胶原基骨/活性肽P17-骨形态发生蛋白2具有良好的骨诱导能力,拔牙后即刻植入可促进拔牙创的愈合,延缓剩余牙槽嵴的吸收。BACKGROUND： Al reasons for tooth extraction are able to cause the alveolar ridge absorption, resulting in jaw bone deficiency, which is harmful to the denture and dental implant repair. OBJECTIVE： Through the establishment of an alveolar ridge model in rats after tooth extraction, to observe the effect of nano-hydroxyapatete/col agen/P17-bone morphogenetic protein-2 on tooth crypt repair and alveolar ridge absorption. METHODS： Thirty-six healthy Sprague-Dawley rats were randomly divided into experimental group, control group and simple material group, and each group had 12 rats. The right mandibular incisors were removed in al the rats. nano-hydroxyapatete/col agen/P17-bone morphogenetic protein-2, nano-hydroxyapatete/col agen/bone morphogenetic protein-2 and nano-hydroxyapatete/col agen were implanted into the tooth crypt of the three groups, respectively. RESULTS AND CONCLUSION： After 2, 4, 8 weeks, alveolar ridge relative length in the experimental group and the control group was more than that in the simple material group （P 〈 0.05）,but there was no difference between the experimental and control groups （P 〉 0.05）. After 2, 4, 8 weeks, the experimental group and the control group had better new blood vessels and new bone formation speed and quality than the simple material group. Percentage of new bone area accounting for bone defect area was increased with time after 2, 4, 8 weeks, which was higher in the experimental and control groups than the simple material group （P 〈 0.05） and higher in the control group than the experimental group. These findings indicate that nano-hydroxyapatete/col agen/P17-bone morphogenetic protein-2 material has good bone induction ability, and dental implant can promote the healing immediately after tooth extraction and delay alveolar ridge absorption.

关 键 词：生物材料 纳米生物材料 拔牙 剩余牙槽嵴 纳米晶矿化胶原 骨形态发生蛋白2 活性肽 新骨形成 拔牙窝 口腔生物材料 生物材料图片文章 

分 类 号：R318[医药卫生—生物医学工程]