人三叶因子3基因启动子区的生物信息学分析  

Bioinformatic analysis of human TFF3 promoter region

作　　者：孙勇[1] 王良喜[1] 孙曙光[1] 王静[1] 毛学飞[1] 邓向东[1] 

机构地区：[1]中国人民解放军第九七医院烧伤整形科,江苏徐州医学博士221004

出　　处：《医学研究生学报》2013年第4期340-342,共3页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81100252);南京军区医学科研课题重点项目(09Z007)

摘　　要：目的人三叶因子3(human trefoil factor 3,hTFT3)是一种具有胃肠黏膜修复和保护作用的小分子多肽,文中利用生物信息学在线软件预测hTFF3基因启动子功能。方法获取hTFF3基因启动子全长序列,利用多种在线相关软件预测出CpG岛及转录因子结合部位。结果 hTFF3基因序列全长为2295 bp,核苷酸数据库(GenBank)登录号为AB038162.1。hTFF3启动子存在于非编码区上游1500bp内,启动子序列中未发现CpG岛,启动子区共发现204个转录因子。结论生物信息学在线软件能预测hTFF3基因启动子功能,提高了针对启动子的研究效率。Objective Human trefoil factor 3 （hTFT3） is a low molecular weight polypeptide with protection and repair func- tion of the gastrointestinal mucosa. The aim of the present study was to predict the function of the promoter region of hTFF3 using online bioinformatics tools. Methods The promoter sequence of hTFF3 was obtained from Genbank, and the CpG islands and transcription factor binding sites were predicted by CpG Island, CpGPlot, Promoter 2.0, Neural Network Promoter Prediction, Promoter SCAN and AliBaba 2.1. Results The full length of the hTFF3 geue sequence, GenBank AB038162.1, was 2295 bp. 1500 bp around tran- seriptional start site might be promoter regions, and the CpG islands didn＇t exist in the promoter region of hTFF3. In addition, total 204 transeription factor binding sites were found. Conclusion Gene promoter related bioinformatics can improve the efficieney of gene promoter research, and provide signifieant information for the prediction of gene promoter funetion.

关 键 词：人三叶因子 启动子 生物信息学 转录因子 

分 类 号：Q78[生物学—分子生物学]