两种可注射磷酸钙骨水泥的细胞相容性研究  

Cell biocompatibility of two injectable calcium phosphate cements

作　　者：孟丹[1] 谢秋菲[2] 肖军军[3] 

机构地区：[1]首都医科大学口腔医学院修复科,北京100050 [2]北京大学口腔医学院 [3]北京大学医学部

出　　处：《北京口腔医学》2013年第2期65-67,共3页Beijing Journal of Stomatology

基　　金：国家自然科学基金资助(30772447)

摘　　要：目的检测可注射α-磷酸三钙/磷酸钙骨水泥和β-磷酸三钙/磷酸钙骨水泥材料的体外细胞相容性。方法将小鼠成骨前体细胞株MC3T3-E1分别接种于α-磷酸三钙/磷酸钙骨水泥和β-磷酸三钙/磷酸钙骨水泥的固化块上,应用扫描电镜观察细胞在材料上的粘附情况。通过四唑盐比色实验(MTT法)和碱性磷酸酶(alkaline phosphatase,ALP)活性测定,检测细胞的增殖、分化能力。结果 MC3T3-E1在α-磷酸三钙/磷酸钙骨水泥和β-磷酸三钙/磷酸钙骨水泥固化块上能较好地粘附,在β-磷酸三钙/磷酸钙骨水泥固化块上MTT吸光值随时间延长增长幅度较大,且ALP活性高于α-磷酸三钙/磷酸钙骨水泥。结论 MC3T3-E1的增殖和分化能力在β-磷酸三钙/磷酸钙骨水泥上高于α-磷酸三钙/磷酸钙骨水泥。Objective To examine the cell biocompafibility of injectable α-TCP/CPC and β-TCP/CPC in vitro. Methods The mouse preosteoblastic cells （ MC3T3-E1 ） were cultured on setting discs of β-TCP/CPC and α--TCP / CPC. The adhesion,proliferation and differentiation of the MC3T3-E1 were observed by SEM, MTY assay and the activity of alkaline phosphatase（ALP）. Results The MC3T3-E1 could adhere on the discs of α-TCP/CPC and β-TCP/CPC, the MTr value of β-TCP/CPC increased rapidly with time and the activity of ALP of β-TCP/CPC was higher than that of α- TCP/CPC. Conclusion The proliferation and differentiation of the MC3TB-E1 on β-TCP/CPC discs were higher than those of α--TCP/CPC.

关 键 词：磷酸钙骨水泥 可注射性 细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程]