稀释曲线法评价TgAb对Tg测定的干扰  

TgAb Interference with Experimental Tg Values Detected by Dilution Curves

作　　者：吴瑕[1] 胥劲[1] 倪建[2] 俞英[3] 张文杰[1] 潘明志[1] 黄蕤[1] 唐恭顺[1] 

机构地区：[1]四川大学华西医院核医学科,成都610041 [2]四川省简阳市人民医院核医学科简阳641400 [3]成都中医药大学附属第二医院内科,成都610041

出　　处：《四川大学学报（医学版）》2013年第3期432-435,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.30970859)资助

摘　　要：目的探讨稀释曲线法评价分化型甲状腺癌(DTC)患者内源性甲状腺球蛋白抗体(TgAb)对甲状腺球蛋白(Tg)测定的干扰。方法用血清稀释液、6例DTC患者可测浓度的TgAb血清(TgAb 20～25IU/mL、Tg<0.1μg/L)、5例DTC患者不可测浓度TgAb血清(TgAb<10IU/mL、Tg<0.1μg/L)分别稀释另外6例DTC患者的Tg血清(TgAb<10IU/mL、Tg>10μg/L),三明治夹心法(IMA)测定各稀释管Tg值。以各稀释管Tg理论值为横坐标、实测值为纵坐标,绘制稀释曲线图。结果血清稀释液稀释DTC患者Tg血清,稀释曲线呈直线。6例可测浓度TgAb血清分别稀释6例DTC患者Tg血清,获得36条稀释曲线,其中12条呈直线,24条不呈直线;同一可测浓度TgAb血清稀释不同患者Tg血清,仅部分呈直线;不同患者TgAb血清稀释同一患者Tg血清,仅部分呈直线。5例不可测浓度TgAb血清稀释3例患者Tg血清,只完成稀释曲线11条,仅4条呈直线,7条不呈直线。结论同一患者的TgAb稀释多例患者的Tg血清,部分有干扰。不可测浓度TgAb也可能干扰其他患者的Tg测定。稀释曲线法仅可间接评价DTC患者自身TgAb是否干扰Tg测定。Objecitve To identify the interference of endogenous antithyroglobulin （TgAb） with experimental thyroglobulin （Tg） values using dilution curves. Methods Dilution buffer, detectable TgAb：serums （TgAb 20-25 IU/mL, Tg free） and undetectable TgAb serums （TgAb 〈10 IU/mL, Tg free） were employed to dilute the Tg serums from patients with DTC, respectively. The Tg values were detected by immunometric assay （IMA）. The experimental Tg values （Y-axis） were plotted against expected serum Tg values （X-axis）. Diluted curves were used to evaluate the interference of TgAb on the experimental Tg values. A linear dilution curve is supposed to appear if no TgAb interference exists. Results The Tg dilution curves with dilution buffer were linear. Thirty six dilution curves were obtained with TgAb serums from six patients diluted by detectable TgAb serums, and 12 showed linear. Tg serums from six patients diluted by one detectable TgAb serum resulted in both linear and non-linear results. One Tg serum diluted by six TgAb serums also resulted both linear and non-linear results. Tg serums from three patients diluted by five undetectable TgAb serums resulted in 11 dilution curves, four of which were linear. Conclusion Dilution curves can be used to predict TgAb interference indirectly. Detectable TgAb may not interfere with experimental Tg values. Whereas, undetectable TgAb may interfere with Tg values. TgAb could not be used to predict Tg interference.

关 键 词：分化型甲状腺癌 甲状腺球蛋白 甲状腺球蛋白抗体稀 释曲线 

分 类 号：R581[医药卫生—内分泌]