儿童过敏性鼻炎特异性免疫治疗过程中IL-10、TGF-β1和sIgE的变化及其与疗效的关系  

Changes in IL-10,TGF-β and sIgE in Children with Allergic Rhinitis Post-immunotherapy and Correlations with Symptoms

作　　者：刘国钧[1] 凡启军[1] 高金建[1] 刘宝林[2] 刘学军[1] 倪丽艳[1] 

机构地区：[1]温州医学院附属第二医院耳鼻咽喉科,325000 [2]温州市中心血站

出　　处：《医学研究杂志》2013年第4期115-117,共3页Journal of Medical Research

基　　金：温州市科技局基金资助项目(Y20110143)

摘　　要：目的研究儿童过敏性鼻炎(allergic rhinitis,AR)特异性免疫治疗(specific immunotherapy,SIT)前及1年后血清IL-10、TGF-β1以及特异性IgE(specific IgE,sIgE)的变化情况,并观察其疗效,初步探讨儿童AR的发病及SIT的治疗机制。方法应用酶联免疫吸附法测定24例儿童AR患者血清IL-10、TGF-β以及sIgE水平,并与24例健康儿童比较,在SIT1年后对以上指标进行再评估,并对患者的症状和体征进行评分。结果 AR患儿SIT1年后,症状和体征评分由治疗前的11.45和3.41分降为治疗后的4.62和1.45分,但患者sIgE的水平无明显降低。治疗前IL-10为3.56pg/ml,明显低于正常对照组儿童的13.37pg/ml(P<0.05),1年后升至11.78pg/ml,接近正常水平(P<0.05)。TGF-β1在正常儿童,AR患儿免疫治疗前、1年后分别为8.97、9.68、24.39pg/ml;AR患儿与正常对照组比显著性差异(P>0.05),但治疗后的水平显著升高(P<0.05)。IL-10、TGF-β1与症状评分均没有相关性(P均>0.05)。结论 AR患儿存在免疫细胞功能下降,导致细胞因子IL-10的分泌明显降低,SIT治疗可以明显改善AR患儿的症状和体征,调节免疫细胞的功能,增加IL-10、TGF-β1的分泌。Objective To research the changes of IL - 10,TGF - 61 and sIgE in the children with allergic rhinitis after one year of specific immunotherapy treatment and correlations with symptoms. Methods We used the peripheral blood from 24 AR patients and 24 healthy subjects as samples to measure the levels of IL - 10, TGF -β1 by enzyme - linked immunoabsorbent assay. After one year of SIT, we assayed the levels again. We also observed the changes of sIgE and the scores of nasal symptom and sign. Results After one year of SIT, the scores of nasal symptom and sign from 11.45 improved to 4.62, as to the scores of sign improved from 3.41 to 1.45 ,but the lev- el of serum slgE was of no change. The level of IL - 10 was 3.56pg/ml in the 24 children with allergic rhinitis, significantly lower than 13.37pg/ml in the 24 healthy children （P 〈0.05）. After one year of SIT, IL - 10 increased to 11.78pg/ml,closed to the normal level（P 〈 0.05 ）. As to TGF - β1 ,the level was 9.68pg/ml,not higher than 8.97pg/ml of the normal（ P 〉 0.05 ）. But after one year of SIT, the level of 24.39pg/ml was significantly higher than the normal and before SIT （P 〈 0.05）. The level of IL - 10 and TGF - β1 showed no correlation with the scores of nasal symptom and sign respectively （ P 〉 0. 05 ）. Conclusion The immunocyte cell functions may decline in the AR children, which result in the decrease of secretion of IL - 10. SIT can improve the symptoms and signs of AR by regulating the im- munocyte cell functions, which increased the secretion of IL - 10 and TGF - β1 .

关 键 词：过敏性鼻炎 特异性免疫治疗 IL-10 TGF—β1sIgE 

分 类 号：R725.622.5[医药卫生—儿科]