黄花夹竹桃中强心苷类成分的UPLC-QTOF/MS快速鉴定  

The rapid indentification of cardiac glycosides from Thevetia peruviana by UPLC - QTOF / MS

作　　者：文屏[1] 郭巧技[1] 黄晓炜[1] 王建文[1] 王春华[2] 

机构地区：[1]深圳市药品检验所,广东深圳518057 [2]天津中医药大学,天津300457

出　　处：《中国民族医药杂志》2013年第4期45-46,共2页Journal of Medicine and Pharmacy of Chinese Minorities

摘　　要：目的:应用超高效液相色谱-高分辨质谱联用方法(UPLC-QTOF/MS)对黄花夹竹桃枝中的强心苷类成分进行速鉴定,为含有黄花夹竹桃的复杂混合样品的快速分析提供了一种有效的方法。方法:黄花夹竹桃枝经甲醇冷浸提取,大孔吸附树脂层析,将黄花夹竹桃中总强心苷类成分富集到95%乙醇洗脱部位,通过超高效液相色谱将各种强心苷类成分较好分离,根据高分辨质谱得到各成分的分子量,推测出其中6个主要成分的可能结构。结果:试验通过UPLC-QTOF/MS方法鉴定出黄花夹竹桃中主含的6种强心苷类成分,分别为黄花夹竹桃黄夹苷B、黄夹次苷D、黄花夹竹桃次苷甲、黄夹臭蚁醛苷、黄花夹竹桃次苷乙以及单乙酰次苷乙。结论:该实验方法简便快捷,结果准确可靠,可做为黄花夹竹桃中强心苷类化合物的快速鉴别分析方法。Objective：To indentify the cardiac glycosides from Thevetia peruvian rapidly by UPLC-QTOF/MS speed. Method：The car- disc glycosides fraction was obtained after quench extraction by methanol and macropemus adsorption resin chromatography, then speculated the 6 main cardiac glycosides by UPLC-QTOF/MS. Results：The 6 main cardiac glycosides were identified as Thevetin B, Perusitin, Peruveside, Theveside, Nefiifolin, Cerberin, respectively accroding to these formula weights. Conclusions： This method is simple, accurate and reliable, could be used for the rapid identification of cardiac glycosides from Thevetia pemviana.

关 键 词：黄花夹竹桃 强心苷 超高效液相色谱-高分辨质谱联用 

分 类 号：R29[医药卫生—民族医学]