还原型谷胱甘肽与纳洛酮对急性乙醇中毒小鼠的催醒作用  

The consciousness -regaining effect of reduced glutathione and naloxone hydrochloride on mice under acute alcohol intoxication

作　　者：王芷[1] 李秋冬[1] 郝婷[1] 葛德高[1] 程慧慧[1] 郭玉磊[1] 文雯[1] 刘莹[2] 武玉清[3] 

机构地区：[1]徐州医学院麻醉学院2009级3班,江苏徐州221004 [2]徐州医学院病理学教研室 [3]徐州医学院麻醉药理学教研室

出　　处：《徐州医学院学报》2013年第3期145-147,共3页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金（81070889,81171013）;江苏省高校自然科学基金重大资助项目（10KJA320052）;国家级大学生创新创业训练计划项目重点项目（201210313006）;江苏省高校大学生实践创新训练计划重点项目（20121824）：江苏高校优势学科建设工程资助项目;江苏省青蓝工程优秀青年骨干教师资助项目.

摘　　要：目的观察还原型谷胱甘肽（GSH）和纳洛酮（NAL）对急性乙醇中毒小鼠的催醒效应。方法将48只小鼠随机分为4组：生理盐水组、还原型谷胱甘肽组、纳洛酮组、联合用药组，每组再分为2组；分别观察预防、治疗给药对急性乙醇中毒小鼠中毒潜伏期、中毒时间的影响。结果GSH、NAL预防给药均不能显著延长乙醇中毒潜伏期；预防给药和治疗给药均能显著缩短中毒时间，且联合给药效果尤著。结论GSH和NAL对急性乙醇中毒小鼠具有协同催醒作用。Objective To observe the consciousness - regaining effect of reduced glutathione （GSH） and naloxone hydrochloride （NAL） on mice under acute alcohol intoxication （AAI）. Methods 48 mice were randomly allocated into 4 groups ： NS group, GSH group, NAL group and NAL ＋ GSH group ; and each group was divided into two groups. The consciousness - regaining effect was observed respectively in a preventive experiment （ delivering the drug prior to alco- hol） and also a treatment experiment （delivering the alcohol prior to the drug）. Main outcome measurement： the time of getting AAI and the lasting time of AAI. Results There was no significant difference in shortening the time of getting AAI among GSH, NAL and GSH ＋ NAL groups. There were significant effects on decreasing the lasting time of AAI in both preventive and treatment experiments. Also in both experiments, NAL ＋ GSH group had a significantly shorter last- ing time of AAI than NAL group or GSH group. There was no significant difference in the time of getting AAI between preventive and treatment experiments. Conclusion Combined use of GSH and NAL has synergistic consciousness - re- gaining effect on mice under acute alcohol intoxication.

关 键 词：还原型谷胱甘肽 纳洛酮 急性乙醇中毒 催醒 小鼠 

分 类 号：R961[医药卫生—药理学]