β3肾上腺素能受体mRNA在肥胖倾向型小鼠附睾脂肪垫中表达上调  

Up-regulation of β3-adrenergic receptor mRNA in epididymal fat pad of lipophilic mice

作　　者：罗雄[1] 唐小军 梅志强[1] 杨蕾[1] 李茂仙[1] 周会松[1] 于海清[1] 

机构地区：[1]泸州医学院医学基础研究中心 [2]泸州医学院附属医院心胸外科,四川泸州646000

出　　处：《泸州医学院学报》2013年第2期111-113,共3页Journal of Luzhou Medical College

基　　金：国家自然科学基金项目(81172237);四川省教育厅项目(12ZA241);四川省卫生厅项目(120379)

摘　　要：目的:观察肥胖倾向型小鼠附睾脂肪垫中β3肾上腺素能受体(β3-AR)mRNA水平。方法:通过高脂饮食诱导肥胖倾向小鼠模型,正常饮食组作为对照(n=8),高脂饮食组(n=8)饲喂5周,鉴定为肥胖倾向型小鼠,获得附睾脂肪垫组织。逆转录和半定量聚合酶链反应(SQ-PCR)法分析β3-AR mRNA的表达,GAPDH mRNA为内参照。结果:肥胖倾向型和非倾向型小鼠脂肪垫β3-AR/GAPDH mRNA比分别为1.98±0.34和1.19±0.26,肥胖倾向型小鼠附睾脂肪垫β3-AR mRNA的表达水平上调具有统计学意义(P<0.01)。结论:β3-AR与脂肪代谢密切相关,可望作为治疗干预人类肥胖和Ⅱ型糖尿病的潜在靶点。Objective： To detect the level of β3-adrenergic receptor （β3-AR） mRNA in epididymal fat pad of lipophilie mice. Methods： Lipophilie mice （n=8） were induced via a high-fat diet. Controlled mice （n=8） fed the standard rodent diet. After five weeks feeding, epididymal fat pads were obtained. The expression of β3-AR mRNA was analyzed by using reverse transcription and semi-quantitative polymerase chain reaction （SQ-PCR） with GAPDH mRNA as internal reference. Results： The fat β3-AR/GAPDH mRNA ratios were 1.98±0.34 and 1.19±0.26 in lipophilic group and control group respectively. The expression level of β3-AR mRNA in epididymal fat pad was significantly increased （P〈0.01） in lipophilic mice. Conclusion： This result suggests that β3-AR mRNA expression was closely related to fat metabolism, which suggested that β3-AR was a potential target for therapeutic intervention in human obesity and type 2 diabetes.

关 键 词：Β3肾上腺素能受体 高脂饮食 附睾脂肪垫 半定量PCR 

分 类 号：Q522.2[生物学—生物化学]