日本血吸虫可溶性成虫抗原和虫卵抗原诱导调节性T细胞能力差异的比较  

Activities of Treg cells stimulated by soluble adult worm antigen and egg antigen of Schistosoma japonicum

作　　者：董潇潇[1] 张萃[1] 杨晓玮[1] 李永[1] 陈晓军[1] 薛雪[1] 张伟伟[1] 徐志鹏[1] 孔文珺[1] 朱继峰[1] 周莎[1] 刘丰[1] 苏川[1] 

机构地区：[1]南京医科大学病原生物学系,南京210029

出　　处：《中国血吸虫病防治杂志》2013年第2期146-150,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(81271861;81071385)

摘　　要：目的观察并比较日本血吸虫可溶性成虫抗原(SWA)与可溶性虫卵抗原(SEA)诱导CD4+CD25+Foxp3+调节性T(Treg)细胞水平及其抑制能力的差异。方法分别以PBS、SWA和SEA免疫小鼠,取小鼠脾淋巴细胞,采用流式细胞术检测各组Treg细胞占CD4+T细胞的比例,以及Treg细胞中产IL 10、TGFβ的细胞比例。采用磁珠分选出各组小鼠的Treg细胞后,以3H TdR掺入法检测其抑制靶细胞增殖的能力。结果与SWA相比,SEA可诱导更多的Treg细胞(P<0.05),刺激Treg细胞免疫抑制功能的能力也更强(P<0.05);SWA、SEA免疫小鼠后,SEA刺激Treg细胞产生IL 10、TGFβ的能力更强(P<0.01)。结论SEA较SWA能更好地活化诱导Treg细胞发挥免疫抑制作用。Objective To observe and compare the effects of soluble adult worm antigen （SWA） and soluble egg antigen （SEA） of Schistosomajaponicum on the induction of Treg cells and the suppressive activity of Treg cells. Method Splenocytes were prepared from mice treated with PBS, SWA, and SEA, respectively, and then the proportions of Treg cells and the levels of IL-10 and TGF-a in Treg cells were determined by FACS. The purified Treg cells from the mice treated as above-mentioned were detected for their immunosuppressive activities by incorporation of [3H] thymidine for the final 16 h of culture. Results Com- pared to SWA, SEA induced the higher proportion of Treg cells with a stronger suppressive activity, which produced the higher levels of IL-10 and TGF-I3 （P 〈 0.05）. Conclusion SEA significantly induces Treg cells and enhances their immunosuppressive activity.

关 键 词：日本血吸虫 可溶性成虫抗原 可溶性虫卵抗原 免疫抑制 调节性T细胞 

分 类 号：R383.24[医药卫生—医学寄生虫学]