3种植物生长调节剂的HPLC分离分析方法  

Study on separation and determination of three kinds of plant growth regulator by HPLC

作　　者：付飞娥[1] 刘光辉[1] 熊杰[1] 王兴红[2] 曹秋娥[1] 

机构地区：[1]云南大学化学科学与工程学院教育部自然资源药物化学重点实验室 [2]云南大学微生物研究所,云南昆明650091

出　　处：《云南民族大学学报（自然科学版）》2013年第3期162-166,共5页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(20965009)

摘　　要：在系统优化了流动相组成、流速及柱温等色谱条件后,采用Waters C18色谱柱(4.6 mm×250 mm,5μm)为固定相,体积比为40∶60的甲醇-水(含0.2%冰醋酸和0.1%四氢呋喃)二元溶剂体系为流动相,在流速为1.0 mL/min,柱温为30℃,进样量为10.0μL,检测波长为210 nm的条件下,建立了1个同时分离测定赤霉素(GA)、6-糠氨基嘌呤(6-KT)和吲哚乙酸(IAA)的高效液相色谱方法.该方法可以在12 min内实现微生物菌体及其发酵液中这3种组分的同时分离测定,相对标准偏差在5.0%以内,回收率在106.5%～91.9%之间.A HPLC method for the simultaneous separation and determination of gibberellin A3 （GA） , indoleacetic acid （IAA） and 6-furfurylaminopurine （6 -KT） was developed after the optimizing of the chromatographical conditions like the composition and concentration of the mobile phase together with the flow rate and column temperature. Gibberellin A3, indoleacetic acid and 6 - furfurylaminopurine in the microbe and its fermentation broth could be separated and determined effectively within 12 min by using Waters C18 （4.6 mm × 250 mm,5 μm） as the column, 40% methanol, 60% aqueous solution containing O. 2% acetic acid and O. 1% tetrahydrofuran as the mobile phase in the flow rate of 1.0 mL/min, the column temperature of 30 ℃ and determination wavelength of 210 rim. The recovery obtained was in the range of 91.9% and 106.5%. The relative standard deviation （RSD） was below 5.0%.

关 键 词：高效液相色谱法 赤霉素 6-糠氨基嘌呤 吲哚乙酸 微生物 

分 类 号：R927.2[医药卫生—药学]