糖肾宁对糖尿病大鼠的肾脏保护作用及其对TGF-β_1/Smad7信号转导通路的影响  

Renal protective effect of Tang Shen Ning capsule on diabetic nephropathy rats and the TGF-β_1/Smad7 signal transduction pathway

作　　者：施鹏[1] 赵宗江[1] 张新雪[1] 杨美娟[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中华中医药杂志》2013年第5期1436-1440,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然基金项目(No.30973831);北京市中医管理局"51510"资助项目(No.JJ2007-006)~~

摘　　要：目的:探讨糖肾宁对链脲佐菌素(STZ)诱导糖尿病肾病(DN)大鼠的肾脏及其TGF-β1/Smad7信号转导通路的影响。方法:Wistar大鼠54只,二次随机分为正常组10只,模型组15只、厄贝沙坦组15只和糖肾宁组14只。2周测大鼠体质量;每4周收集大鼠24h尿液,考马斯亮蓝G-250法测24h尿蛋白定量。14周时处死大鼠,检测血清尿素氮(BUN)、血清肌酐(Scr)、甘油三脂(TG)、一氧化氮(NO)、丙二醛(MDA)、超氧化物歧化酶(SOD);大鼠肾组织行HE、Mallory染色并行病理学评分;免疫组化、原位杂交检测肾组织TGF-β1、Smad7、NF-κB蛋白及其mRNA表达。结果:糖肾宁可以改善DN大鼠的一般状况,升高DN大鼠体质量及降低肾质量体质量比(P<0.01),明显降低24h尿蛋白定量(P<0.01),减轻肾组织病理性损害。与模型组比较,糖肾宁组BUN、Scr、TG、MDA含量显著降低(P<0.01),NO、SOD含量明显升高(P<0.01);肾组织TGF-β1、NF-κB蛋白及mRNA表达明显降低(P<0.01),Smad7蛋白及mRNA表达显著升高(P<0.01)。结论:糖肾宁可能通过调控TGF-β1/Smad7信号转导通路,减轻肾脏病理损害而保护肾功能,进而延缓其病程进展。Objective： To discuss the renal protective effect of Tang Shen Ning capsule on diabetic nephropathy rats （DN rats） induced by streptozotocin （STZ） and the TGF-β1/Smad7 signal transduction pathway. Methods： 54 Wistar rats were randomly divided into the follow groups for twice： the normal control group （n=10）, the model group （n=15）, the irbesartan group （n=15）, and the Tang Shen Ning group （n=14）. The body weight at an interval of 2 weeks; 24 hours urine by collecting every four- week intervals was detected its protein content by coomassie brilliant blue G-250 method. Rats were executed on the 14th week. BUN, Scr, TG, MDA, NO and SOD in the blood serum were tested; histopathological scores and the HE, Mallory staining ways were applied to the kidney tissues. Via immunohistochemistry and in situ hybridization, the renal protein of TGF-β1, Smad7, NF- kB and their mRNA expressions were detected. Results： The general condition of rats could be improved by Tang Shen Ning capsules; the weight of DN rats could be increased and the kidney weight/body weight （KW/BW） could be reduced （P〈0.01）; the 24 hours urinary protein were significantly reduced（P〈0.01）; pathological damage of kidney could be relieved. Compared with the model group, the contents of BUN, Scr, TG, MDA could be obviously reduced （P〈0.01）, and NO, SOD increased apparently （P〈0.01）; the expressions of TGF-β1, NF-kB and their mRNA were significantly reduced （P〈0.01）, while Smad7 and its mRNA could be increased （P〈0.01）. Conclusion： It was found that Tang Shen Ning capsule had the function of protecting kidney, through regulating the expression of the TGF-β1/Smad7 signal transduction pathway. And it could put off the progress of DN rats.

关 键 词：糖肾宁 糖尿病肾病 TGF-Β1 Smad7信号转导通路 

分 类 号：R285.5[医药卫生—中药学]