ZT、KT和2,4-D对香蕉胚性愈伤组织诱导的影响  

Effect of ZT,KT and 2,4-D on Induction of Embryogenic Callus from Banana(Musa spp.)

作　　者：郭畅[1,2] 杨微[2,3] 林贵美[1] 黄素梅[1] 

机构地区：[1]广西农业科学院生物技术研究所,广西南宁530007 [2]广西作物遗传改良生物技术重点开放实验室 [3]广西农业科学院经济作物研究所

出　　处：《现代农业科技》2013年第8期57-58,61,共3页Modern Agricultural Science and Technology

基　　金：广西农业科学院科技发展基金项目(桂农科2011JM11);广西农业科学院基本科研业务专项(桂农科2012YM23)

摘　　要：以香蕉未成熟雄花序为外植体,以MS为基本培养基,对外植体进行表面消毒、不同切割长度处理、不同植物生长调节剂(ZT、KT、2,4-D)在不同浓度情况下进行胚性愈伤组织诱导的试验。结果表明:外植体表面消毒以选用10%H2O2溶液处理效果最好,成功率为83.3%;切割长度以1.0 cm较为合适;胚性愈伤组织诱导最适培养基为MS+0.5 mg/L ZT+0.5 mg/L KT+4.0 mg/L 2,4-D,胚性愈伤组织诱导率为12.3%。The banana immature male flowers were used as the explants for embryogenic callus induction in this study.Surface sterilized explants, length of explants and concentration of ZT, KT,2,4-D were researched in MS medium.The results showed that the most suitable surface sterilized explants were 10% H202 treatment, the success rate was 83.3%, explants were sectioned as 1.0 cm length. The best inducing medium for embryogenic callus induction was MS medium with 0.5 mg/L ZT ,0.5 mg/L KT and 4.0 mg/L 2,4-D ,frequency of embryonic callus induction was 12.3%.

关 键 词：香蕉 胚性愈伤组织 诱导 

分 类 号：S668.1[农业科学—果树学]