山东省番茄灰霉病菌种类与遗传多样性研究  

The Study on Species and Genetic Diversity of Botrytis cinerea Populations in Shandong Province

作　　者：李廷刚[1] 张路[1] 周善跃[1,2] 李敏[3] 李宝笃[1,2] 

机构地区：[1]青岛农业大学农学与植物保护学院,山东青岛266109 [2]山东省植物病虫害综合防控重点实验室,山东青岛266109 [3]青岛农业大学园林园艺学院,山东青岛266109

出　　处：《中国农学通报》2013年第10期137-143,共7页Chinese Agricultural Science Bulletin

基　　金：国家"十二五"科技支撑计划项目"北方设施蔬菜高效节能集约化育苗技术研究与示范"(2011BAD12B03)

摘　　要：为明确山东省番茄灰霉病菌的类型分布、遗传多样性以及遗传群组的划分与地理来源之间相关性。采用形态学观察结合5.8SrDNA-ITS序列分析法对单孢分离所得66个菌株进行了鉴定,利用ISSR标记技术对菌株进行遗传多样性分析,并采用UPGMA法进行聚类统计。鉴定结果显示,66个菌株均为灰葡萄孢菌(Botrytiscinerea),通过序列比对,该66个菌株的ITS序列属于2种类型,并且2种ITS序列只有单碱基差异。ISSR遗传多样性分析,结果获得24个ISSR分子标记,其中58.33%的片段具有多态性,UPGMA聚类分析将66个菌株划分为4个遗传聚类群。以上结果表明,菌株种类与遗传多样性均不丰富,遗传群组的划分与菌株的地理来源存在一定的相关性。In order to know the type distribution, the genetic diversity and the correlation between genetic group division and geographical origin of Botrytis cinerea in Shandong Province. In this paper, the morphological observation combined with 5.8S rDNA-ITS sequence analysis was used to identify the 66 isolations that obtained through the method of single spore, and the genetic diversity was analyzed by the inter-simple sequence repeats （ISSR）, at the same time the cluster statistics was analyzed with UPGMA method. Appraisal results showed that 66 isolates were all the Botrytis cinerea. In addition, all the ITS sequences of 66 isolates were divided into 2 types through the sequence alignment, and only a single base differences between 2 types. Through ISSR genetic diversity analysis 24 sites were generated in total among which 58.33% were polymorphic. By UPGMA cluster analysis, the isolates were classified into 4 subgroups. All the above results indicated that strain types and kinds of genetic diversity were not rich, genetic group division and strain geographical source had certain relevance.

关 键 词：番茄灰霉病 灰葡萄孢菌 ITS 遗传多样性 ISSR 

分 类 号：S4[农业科学—植物保护]