miR-26a和miR-30d在牛不同组织中表达的规律分析  

Analysis on the Expression Regulation of miR-26a and miR-30d in the Different Tissues of Cattle

作　　者：孙广杰[1,2] 戴立胜[2] 袁宝[2] 朱亚南[1,2] 张阳阳[1,2] 柳思源[1,2] 任久生[1,2] 徐靖博[1,2] 李付娟[1,2] 张嘉保[2] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]吉林大学实验动物中心,长春130062

出　　处：《中国农学通报》2013年第11期29-33,共5页Chinese Agricultural Science Bulletin

基　　金：现代农业产业技术体系建设专项资金"国家肉牛牦牛产业技术体系胚胎工程岗位"(CARS-38)

摘　　要：检测miR-26a和miR-30d在牛不同组织中的相对表达水平,并应用生物信息学方法分析其靶基因,探讨两者在牛不同组织中可能发挥的作用。以西门塔尔牛为研究对象,利用TRIzol一步法提取牛各组织总RNA后,根据miRBase提供的bta-miR-26a和bta-miR-30d成熟序列,设计特异性的反转录茎环引物以及荧光定量PCR引物,反转录获得cDNA后进行荧光定量PCR检测其在牛不同组织中的相对表达水平,并应用Targetscan、RNA22、miRDB等生物学软件预测其可能存在的靶基因。miRNA-26a和miR-30d在牛心脏、肝脏等组织中均有不同程度的表达,miR-26a在心脏中表达量最高,其次在脾脏、睾丸中的表达量最低。miR-30d在肾脏中表达量最高,其次在心脏、睾丸中表达量最低。通过生物信息学方法分析miRNAs的靶基因,推测miR-26a和miR-30d可能参与调控机体的免疫、繁殖等活动。The current research aimed to study the relative expression levels of miR-26a and miR-30d in the different tissues, and applying bioinformatic methods to analyze the possible role in cattle. Total RNA was extracted by TRIzol in the different tissues of Simmental cattle. The relative expression levels of miR-26a and miR-30d were performed by RT-qPCR with specific stem-loop primers based on the mature sequence of bta-miR-26a and bta-miR-30d. The target gene of bta-miR-26a and bta-miR-30d were predicted by Targetscan, RNA22, miRDB. MiRNA-26a and miR-30d all had certain expression level in all kinds of detected tissues, of which miR-26a had the highest expression level in the heart, followed in the spleen, and lowest level in the testis; miR-30d had the highest expression level in the kidney, followed in the heart, and lowest level in the testis. Bioinformatics showed that miR-26a and miR-30d may be involved in the regulation of the immune, reproductive activities and so on.

关 键 词：MIRNA 实时荧光定量PCR 靶基因 

分 类 号：S823.8[农业科学—畜牧学]