萹蓄中杨梅苷和槲皮苷的含量测定  

Determination of Myricetrin and Quercitroside in Polygonum Aviculare L.

作　　者：林洁[1] 李志浩[1] 陈银华[2] 

机构地区：[1]湖北医药学院附属东风医院,湖北十堰442001 [2]湖北省十堰市药品检验所,湖北十堰442000

出　　处：《儿科药学杂志》2013年第5期46-48,共3页Journal of Pediatric Pharmacy

摘　　要：目的:采用反相高效液相色谱法(RP-HPLC)分析萹蓄中杨梅苷和槲皮苷的含量。方法:Agilent LC-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%磷酸溶液(18︰82)作为流动相,流速为1.0 mL/min,柱温30℃,检测波长256 nm。结果:杨梅苷和槲皮苷分别在1.22～24.30μg/mL和0.77～15.40μg/mL范围内有良好的线性关系,平均加样回收率分别为98.9%、99.6%,RSD分别为1.11%和1.09%。结论:该方法准确,简便,重复性好,可用于萹蓄中杨梅苷和槲皮苷的定量分析。Objective： To establish the RP-HPLC method for the determination of myricetrin and quercitroside in Polygonum aviculare L.. Methods： The Agilent LC-C18（250 mm×4.6 mm, 5μm） column was used, and the mobile phase consisted of acetonitrile： O. 1% H3PO4（18 ： 82）. The flow rate was 1.0 mL/min. The column temperature was 30 ℃. The detecting wavelength was at 256 nm. Results： The calibration curves of myricetrin and quercitroside were linear within a range of 1.22 - 24.30 μg/mL and 0.77 - 15.40 μg/mL. The average recoveries of myricetrin and quercitroside of this method were 98.9% and 99.6% , and the RSD were 1.11% and 1.09%, respectively. Conclusions： The method is simple, repeatable and accurate. It can be applied in quantitative determination of myricetrin and quercitroside in Polygonum aviculare L..

关 键 词：萹蓄 杨梅苷 槲皮苷 高效液相色谱法 

分 类 号：R284[医药卫生—中药学] R927.2[医药卫生—中医学]