麦芽酚铝对大鼠在体海马长时程增强影响  

Effect of aluminum-maltolate complex on long-term potentiation in rat hippocampus in vivo

作　　者：宋静[1] 张慧芳[1] 刘莹[1] 牛侨[1] 

机构地区：[1]山西医科大学公共卫生学院劳动卫生教研室,山西太原030001

出　　处：《中国公共卫生》2013年第5期688-691,共4页Chinese Journal of Public Health

基　　金：国家自然科学基金(30972512);国家青年科学基金(81202182)

摘　　要：目的探讨急性麦芽酚铝[Al(mal)3]染毒对大鼠海马CA1区长时程增强(LTP)影响。方法对照组及低、中、高染铝组大鼠通过侧脑室给药方式分别一次性注射生理盐水、2.43、12.15、60.75μg Al(mal)3,给药容积为5μL,5 min内缓慢匀速注入;采用在体海马CA1区LTP记录技术,记录兴奋性突触后电位(fEPSP)。结果高剂量染铝组大鼠在给药前和给药后30 min时fEPSP分别为(109±4)%和(111±7)%,差异无统计学意义(P>0.05);在高频刺激后60 min时,低、中、高染铝组fEPSP分别为(157±8)%、(140±13)%、(110±7)%,均低于对照组的(190±27)%,而相同剂量下的Al(mal)3对双脉冲易化(PPF)无影响。结论 Al(mal)3剂量依赖性抑制大鼠海马CA1区的LTP诱导和维持,其作用机制可能与突触前机制无关,而与突触后机制有关。Objective To study the effect of aluminum-maltolate （ Al[ real ] 3 ） complex on long-term potentiation （LTP） in rat hippocampus in vivo. Methods The rats were exposed to Al （mal） 3 by acute intracerebroventricular injection. Field excitatory postsynapic potenial（fEPSP） in CAI region of hippocampus was recorded by field potentiation technique in vivo. Results The basal fEPSP of high dose AI（mal） 3 （60. 75 μg） groups with preinjection and postinjection were 109 ± 4% and 111 ± 7 %, respectively, without significant difference （ P 〉 0. 05 ）. In 60 min-post high frequency stimulation, the fEPSP of 2.43 μg, 12. 15 μg, and 60.75 μg groups were 157 ± 8%, 140 ± 13%, and 110 ± 7%, with significant differences compared to that of the control group. But under the same dose of exposure ,AI（real） 3 did not affect paired pulse facilitation（PPF） ratio. Conclusion Al （mal）3 obviously suppresses the LTP in rat hippocampal CAl region in a dose-dependent manner in vivo, probably through postsynaptic mechanism but not presynaptic transmitter release.

关 键 词：麦芽酚铝[Al(mal)3] 长时程增强(LTP) 侧脑室注射 海马 在体 

分 类 号：R134[医药卫生—劳动卫生]