黏菌素对小鼠神经行为功能影响  

Effects of colistin on neurobehavior in mice

作　　者：代重山[1] 李继昌[1] 李健 刘洋 

机构地区：[1]东北农业大学动物医学学院药理与毒理教研室,黑龙江哈尔滨150030 [2]Facility for Anti-infective Drug Development and Innovation,Monash Institute of Pharmaceutical Sciences Monash University

出　　处：《中国公共卫生》2013年第5期701-703,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(31272613);教育部留学回国人员科研启动基金(41);哈尔滨市科技创新人才研究专项资金留学回国人员项目(2012RFLXN005);黑龙江省教育厅科学技术项目(12521043)

摘　　要：目的探讨黏菌素对小鼠运动及感觉功能影响。方法 40只昆明系雌性小鼠分为硫酸黏菌素高、中、低剂量组(静脉注射15.0、7.5、5.0 mg/kg)和对照组(等量生理盐水),每组10只,连续给药7 d,并于第1、3、7、15 d进行神经行为学测试。结果高剂量黏菌素组小鼠体重明显降低;给药后3、7、15 d,高剂量黏菌素组小鼠热痛觉阈值分别为(56.50±7.32)、(72.50±16.30)、(54.75±4.11)s,均长于对照组的(41.33±9.87)、(39.50±8.06)、(38.33±7.23)s(P<0.01);高剂量黏菌素组小鼠在给药后7、15 d,平衡力指数分别为(162.66±11.01)、(180.43±10.12)s,均低于对照组的(200.60±9.02)、(215.00±8.66)s(P<0.01);给药后7 d,高剂量黏菌素组小鼠后肢撑力指数增大至(3.71±0.08)cm,高于对照组的(3.54±0.12)cm(P<0.05)。结论黏菌素可引起小鼠热觉传导异常、运动神经损伤和运动协调能力降低。Objective To investigate the neurotoxic effect of intravenous colistin on mice motor and sensory functions.Methods Forty female Kunming mice were randomly divided into high-dose group（15 mg/kg）,moderate-dose group（7.5 mg/mg）,low-dose group（5 mg/kg）,and control group（0.9% saline）（10 in in each group）.Colistin sulfate was administered intravenously for 7 days and neurobehavioral test were conducted in the mice on 1,3,7,and 15 day after treatment.Results Compared with the control group,the body weight decreased in the mice treated by high-dose colistin.On the day 3,7,and 15,the thermal withdrawal thresholds were 56.5±7.32,72.5±16.3,and 54.75±4.11 s in high-dose group,which were higher than that of the control group（P0.01 for all）.On the day 7 and 15,the balance time were 162.66±11.01 and 180.43±10.12 s in high-dose group,which were lower than that of the control group（P0.01）.On the day 7,the hind limb landing foot splay was increased to 3.71±0.075 cm in high-dose group,which was significantly higher than that of the control group（P0.05）.Conclusion The results confirm that colistin could induce abnormal conduction of heat,motor nerve damage and decreased coorrdination ability of muscles in mice.

关 键 词：黏菌素 运动功能 感觉功能 小鼠 

分 类 号：R978.16[医药卫生—药品]