静电纺丝法制备聚苯胺/醋酸纤维素膜修饰电极  

Preparation of PANI/CA film modified electrode by electro-spinning and its application in biosensor

作　　者：万其进[1] 舒好[1] 尚艳利[1] 许佳[1] 廖华玲[1] 杨年俊[1] 

机构地区：[1]武汉工程大学化工与制药学院绿色化工过程教育部重点实验室湖北省新型反应器与绿色化学工艺重点实验室,湖北武汉430074

出　　处：《武汉工程大学学报》2013年第4期21-25,共5页Journal of Wuhan Institute of Technology

基　　金：国家自然科学基金(21075096;21275113);武汉工程大学研究生教育创新基金项目(CX201112)

摘　　要：利用聚苯胺和醋酸纤维素为原料,按一定的配比配制成溶液,通过静电纺丝法修饰于铂电极表面,制备成聚苯胺/醋酸纤维素(PANI/CA)纳米纤维薄膜修饰电极(PANI/CA/Pt).采用各种电化学方法和扫描电镜(SEM)对PANI/CA纳米纤维薄膜进行了表征,并且用交流阻抗法分析了其在电极表面的动力学过程.结果表明PANI/CA纳米纤维薄膜电化学性质稳定,该修饰电极在H2SO4溶液中呈现出聚苯胺的特征峰,其SEM图显示PANI/CA纳米纤维在电极表面呈网状不规则立体分布,为构建生物传感器提供了一个良好的界面.以此为基础制备的葡萄糖氧化酶/聚苯胺/醋酸纤维素(GOx/PANI/CA/Pt)传感器对葡萄糖有良好的响应,有望制成物美价廉的生物传感器.利用静电纺丝法制备纳米纤维薄膜修饰电极并用来固定酶等蛋白质类高分子物质是一种新的可行性的方法.Polyaniline and cellulose acetate were used as materials, and formulated into a solution at a certain ratio, and electrostatic spinning was used to prepare polyaniline/cellulose acetate （PANI/CA） nano-fiber membrane which was modified on the surface of Pt electrode to prepare the PANI/CA/Pt. PANI/CA nano-fiber film was characterized by electrochemical methods and scanning electron microscopy（SEM）. The results indicate that electrochemical properties of PANI/CA nano-fiber film are stable, and SEM images show their meshy irregular stereoscopic distribution on electrode surface. The electrode modified with nano-fiber film shows polyaniline characteristic peaks in H2 SO4 solution and its dynamic process on the electrode surface is investigated with AC impedance. The prepared GOx/PANI/ CA/Pt modified electrode has a good response to glucose, which is expected to be a good and inexpensive biosensor. It is a truly original and feasible method to use the nanofibers film modified electrodes prepared by the Electrostatic Spinning method to immobilize polymer substances such as enzyme and protein.

关 键 词：静电纺丝 聚苯胺 醋酸纤维素 化学修饰电极 

分 类 号：O657.1[理学—分析化学]