日本血吸虫单克隆抗独特型抗体NP30对虫卵肉芽肿免疫调节作用的研究  

STUDY ON IMMUNOREGULATION OF EGG GRANULOMAS BY MONOCLONAL ANTI-IDIO-TYPIC ANTIBODY NP30 OF SCHISTOSOMA JAPONICUM IN MICE

作　　者：冯振卿[1] 朱荣[1] 李玉华[1] 仇镇宁[2] 李芸茜[2] 冷静[1] 薛婉芬[1] 吴观陵[2] 管晓虹[2] 

机构地区：[1]南京医科大学病理学教研室,南京210029 [2]南京医科大学寄生虫学教研室

出　　处：《中国血吸虫病防治杂志》2000年第4期193-197,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金!(39570645)

摘　　要：目的研究日本血吸虫单克隆抗独特型抗体ＮＰ３０对虫卵肉芽肿的影响。方法 ＮＰ３０腹腔注射主动免疫ＩＣＲ小鼠，对照组腹腔注射ＳＰ２／０腹水，分别在尾蚴攻击感染后第４、８、１２、１６、２０、２４周处死，观察和比较肝内虫卵肉芽肿的形成和演变。应用计算机图像分析技术，测量虫卵肉芽肿大小。结果尾蚴攻击感染第 １２周以后， ＮＰ３０免疫组虫卵肉芽肿的直径明显低于 ＳＰ２／０对照组，虫卵肉芽肿的演变时相提前，并出现２种特殊类型的肉芽肿。结论ＮＰ３０能减轻日本血吸虫对宿主造成的免疫病理损害。Objective To study the immunoregulative effect of the monoclonal anti-idiotypic antibody NP30 of Schistosoma japonicum on egg granulomas. Methods ICR mice were actively immunized with NP30 intraperitoneally, while the control group was injected with SP2/0 ascites intraperitoneally. The mice were killed at the 4th、 8th、 12th、 16th、　20th、 24th week after infection re spectively. The formation and evolution of egg granulomas in liver were observed and compared. The size of egg granulomas was measured by NYD-1 000 Image Analysis System. Results The di ameter of egg granulomas in NP30 immunized group was much lower than that in SP2/0 ascites in jected group after the twelfth week of cercariae challenge. The evolution process of egg granulomas in NP30 immunized group was advanced. In addition, two special types of egg granulomas appeared in the liver. Conclusions NP30 may act as an effective inhibitor to egg granulomas of Schistosoma japonicum and reduce immunopathologic damage caused by Schistosoma japonicum in the host.

关 键 词：日本血吸虫 单克隆抗独特型抗体 虫卵催芽肿 

分 类 号：R532.21[医药卫生—内科学]