滴金免疫测定法检测抗体在血吸虫病传播阻断地区的应用  

THE APPLICATION OF DOT IMMUNOGOLD FILTRATION ASSAY IN THE TRANSMISSION BLOCK AREAS OF SCHISTOSOMIASIS JAPONICA

作　　者：施晓华[1] 汤益[1] 张素娥[1] 蒋健敏[1] 朱明东 任启彰 方海松 

机构地区：[1]浙江省医学科学院寄生虫病研究所,杭州310013 [2]浙江省东阳市卫生防疫站

出　　处：《中国血吸虫病防治杂志》2000年第4期212-213,共2页Chinese Journal of Schistosomiasis Control

摘　　要：目的探讨滴金免疫测定法检测抗体在血吸虫病传播阻断地区的应用价值。方法在已建立的滴金免疫测定法的基础上，应用该法检测原血吸虫病重度流行区、中度流行区和轻度流行区而现已阻断传播地区的人群以及现血吸虫病中度流行区人群的抗血吸虫抗体。结果原血吸虫病重、中和轻度流行区人群的阳性检出率分别为６．１８％、３．３１％、１．４７％，三者间有非常显著差异（Ｐ＜０．０１）；现血吸虫病中度流行区人群的阳性检出率为 ３９．３３％，与传播阻断地区人群的检出率间也有非常显著差异（Ｐ＜０．０１）。结论摘金免疫测定法检测抗体可作为考核该类地区血吸虫病防治效果的一种方法。Objective To investigate the application value of dot immunogold filtration assay (DIGFA) in the schistosomiasis transmit-blocking area. Methods On the basis of developed DIGFA to detect anti-schistosome antibody, the population from original heavy epidemic region, original middle epidemic region, original slight epidimic region and present middle epidemic region were de tected by DIGFA. Results The positive rates of the population from original heavy epidemic re gion,original middle epidemic region and original slight epidemic region were 6.18%, 3.31% and 1.47% , respectively. There were significant differences compared with each other (P<0.01). The positive rate of present middle epidemic region was 39.33%, which was significantly different from that of transmit-blocking region (P<0.01). Conclusion Anti-schistosome antibody detected by DIGFA can be used to evaluate the schistosomiasis control effect in the transmit-blocking area.

关 键 词：滴金免疫测定法 ELISA 血吸虫病 抗体 

分 类 号：R532.21[医药卫生—内科学] R446.62[医药卫生—临床医学]