荧光碳点对人血淋巴细胞标记成像  

Labeling of Human T-Lymphocyte by Fluorescent Carbon Dots

作　　者：董微[1] 王莹[2] 宋有涛[2] 袁贵丽[3] 高艳艳[3] 张爱爱[3] 

机构地区：[1]沈阳医学院化学教研室,沈阳市黄河北大街146号110034 [2]辽宁大学生命科学院,沈阳市110036 [3]沈阳医学院公共卫生学院

出　　处：《光谱实验室》2013年第3期1035-1038,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：辽宁省自然基金资助项目(20102221);辽宁省教育厅资助项目(L2010542);辽宁省教育厅杰出青年学者成长计划(LJQ2011109);沈阳市科技局资助项目(F11-262-9-21);沈阳医学院优秀人才项目(No.20113068)

摘　　要：以葡萄糖为碳源,采用溶剂热法合成荧光碳点。用紫外-可见分光光度法和荧光光谱法研究了荧光碳点的发光特性。并将其与鼠抗人CD3抗体、羊抗鼠IgG抗体连接制备两种水溶性复合探针,对人血淋巴细胞进行免疫荧光标记成像。结果表明用该探针对人血淋巴细胞成像清晰,72h后细胞仍然保持明亮的荧光。Fluorescent carbon dots （CDs） were synthesized by a solvothermal method with glucose as the carbon source and studied by UV-Vis absorption and fluorescence emission spectra. The as-prepared CDs were linked to mouse anti-human CD3 and immunoglobulin （IgG） for the labeling of fixed Humun T-lymphocyte. Experimental results have shown that CDs-based probes are excellent biomarkers for T-lymphocyte and the fluorescent microscopical images of human T-lymphocyte still exhibited bright fluorescence after 72 hours.

关 键 词：荧光碳点 免疫荧光标记 人血淋巴细胞 

分 类 号：O657.32[理学—分析化学]