利福平磷脂复合物的含量测定及光谱学分析  

Determination of Rifampicin-Phospholipid Complex

作　　者：何丹[1] 杨林 滕永真[1] 孙全[1] 张景勍[1] 

机构地区：[1]重庆医科大学高校药物工程研究中心和生物化学与分子药理重点实验室,重庆市渝中区医学院路1号400016 [2]重庆市食品药品检验所,重庆市北部新区春兰二路1号401121

出　　处：《光谱实验室》2013年第3期1107-1111,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：教育部博士点基金资助项目(20095503120008);重庆市教育委员会资助项目(首批高等学校优秀人才资助;KJ090308)

摘　　要：建立紫外分光光度法测定利福平磷脂复合物含量的方法。以无水乙醇为空白,测定波长为474nm。结果表明,在9.842—39.36μg.mL-1范围内利福平的吸光度线性关系良好(r=0.9998),平均回收率为99.89%,RSD为0.57%(n=9)。本方法操作简便,灵敏度高,重现性好,能准确测定利福平磷脂复合物中利福平的含量。To establish a UV Spectrophotometry method for the determination of content of rifampicin-phospholipid complex. The content was determined at 474nm with dehydrated alcohol asthe blank control by ultraviolet spectrophotometry. The results showed that rifampicin had a good linear relationship within a range of 9. 842-39. 36gg · mL-1（r=0. 9998） and average recovery was 99. 89% ,RSD% 0.57 % （n= 9）, This method is simple, accurate and reproducible and can be used as a method for the determination of rifampicin acurally.

关 键 词：利福平 紫外分光光度法 利福平磷脂复合物 红外光谱 

分 类 号：O657.32[理学—分析化学] O657.33[理学—化学]