紫外分光光度法测定姜黄素磺丁基醚-β-环糊精包合物的含量  

Content Determination of Curcumin SBE-β-cyclodextrin Inclusion Complex by UV Spectrophotometry

作　　者：杨梅[1] 胡雪原[1] 万坤[1] 王红[1] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室,重庆市渝中区医学院路1号400016

出　　处：《光谱实验室》2013年第3期1238-1242,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：重庆市教育委员会资助项目(KJ120321)

摘　　要：建立测定姜黄素磺丁基醚-β-环糊精包合物中利福平含量的方法。采用紫外分光光度法测定含量,测定波长为426nm。姜黄素在426nm处有最大吸收,在1.05—7.35μg/mL范围内与吸光度呈良好的线性关系,回归方程为:A=0.1309C+0.0306(r=0.9991);平均回收率为99.40%(n=9),RSD为0.69%;测得姜黄素-磺丁基醚-β-环糊精的包合率为91.71%,RSD为0.38%。本方法操准确、简便、迅速,适用于姜黄素磺丁基-β-环糊精包合物中姜黄素含量的测定。A method for determination of the content of curcumin sulfobutyl ether-β- cyclodextrin inclusion complex was established. The drug concentration in curcumin sulfobutyl ether- β-cyclodextrin inclusion complex was detected at the wavelength of 426nm by using UV-visible spectrophotometry. The curcumin had a good liner relationship range from 1.05-7. 35μg/mL. The regression equation was as follows ： A= 0. 1309C＋ 0. 0306 （r= 0. 9991 ）. The average recovery rate was 99. 40%, RSD= 0. 69M（n = 9）. The complexation rate of Curcumin sulfobutyl ether -fl- cyclodextrin was 91.71%, RSD= 0. 38 %. The method was simple, rapid and accurate,which can be used for determination of the content of curcumin sulfobutyl ether-β-cyclodextrin inclusion complex.

关 键 词：姜黄素 姜黄素磺丁基-β-环糊精包合物 紫外分光光度法 

分 类 号：O657.32[理学—分析化学]