两种二苯醚类除草剂在直链淀粉液相色谱手性固定相上的拆分  

Enantiomeric Separation of Diphenyl Ether Herbicides by HPLC on Amylase Chiral Stationary Phase

作　　者：贾瑞琳[1] 牟仁祥[1] 

机构地区：[1]中国水稻研究所农业部稻米及制品质量监督检验测试中心,杭州市体育场路359号310006

出　　处：《光谱实验室》2013年第3期1345-1349,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：浙江省自然科学基金(Y5090085)

摘　　要：以硅胶涂敷直链淀粉-三(5-氯-2-甲基苯基氨基甲酸酯)为手性固定相,对两种含有1个手性中心的二苯醚类除草剂2-甲氧基-2-{5-[2-氯-4-(三氟甲基)苯氧基]-2-硝基苯}丙酸甲酯和乳氟禾草灵,进行了色谱分离。考察了流动相中异丙醇和正己烷的体积比对乳氟禾草灵对映体分离的影响。结果显示,这2种二苯醚类除草剂可以同时在Chiralpak AY-H色谱柱上获得较好的手性拆分结果;并对农药对映体与固定相之间的保留机理进行了讨论。A high performance liquid chromatographic （HPLC） method using amylose tris-（5- chloro-2-methylphenycarbamate） as chiral stationary phase for the separation of diphenyl ether herbicides enantiomers was developed. The method was based on normal phase model with n-heptane and isopropanol as the mobile phase. The influences of the volume ratio of n-heptane and isopropanol on the separation of diphenyl ether herbicides enantiomers were investigated. The results show that two compounds are well enantiomeric separated each other. The chiral recognition mechanism of diphenyl ether herbicides is discussed by the chromatographic theory.

关 键 词：高效液相色谱 直链淀粉手性固定相 二苯醚类手性除草剂 手性分离 

分 类 号：O658[理学—分析化学] O657.72[理学—化学]