5-溴水杨基荧光酮-曲拉通X-100-羟丙基-β-环糊精荧光猝灭法测定痕量锰  

Fluorescence Quenching Method for Determination of Trace Manganese with 5-Bromo-Salicyl-Fluorone-Triton X-100-Hydroxypropyl-β-Cyclodextrin

作　　者：高金霞[1] 赵福阳[1] 刘嘉祺[2] 李淼晶[1] 崔新宇[1] 

机构地区：[1]牡丹江医学院公共卫生学院,黑龙江省牡丹江市通乡街3号157011 [2]牡丹江医学院药学院,黑龙江省牡丹江市157011

出　　处：《光谱实验室》2013年第3期1434-1436,共3页Chinese Journal of Spectroscopy Laboratory

基　　金：黑龙江省自然科学基金QC2012C072;牡丹江医学院项目(2011-21)

摘　　要：在羟丙基-β-环糊精(HP-β-CD)和曲拉通X-100(Triton X-100)存在下,以NaAc-HAc缓冲溶液为介质,5-溴水杨基荧光酮(5-BrSAF)为荧光试剂,建立了用荧光猝灭法测定痕量锰的新方法。研究了缓冲溶液、显色剂用量、表面活性剂对体系的影响,确定了最佳实验条件。体系的最大激发波长λex=375nm,最大发射波长λem=535nm,Mn2+含量在0.026—3.5μg/25mL范围内与荧光猝灭值ΔF呈线性关系,方法检出限为1.02μg/L。已用于面粉中锰的测定。A new fluorescence quenching method for determination of trace manganese with 5- bromo-salicyl-fluorone （5-BrSAF） in NaAc-HAc medium and in the presence of TritonX-100 and Hydroxypropyl-β-cyclodextrin （HP-β-CD） has been described. The excitation wavelengths were 535nm. The emission wavelengths were 375 nm. The linear range was 0. 026-3. 5μg/25mL. The detection limit was 1.02μg/L. The method has been applied to the determination of trace manganese of flour.

关 键 词：锰 5-溴水杨基荧光酮 羟丙基-Β-环糊精 曲拉通X-100 荧光猝灭法 

分 类 号：O657.32[理学—分析化学]