HPLC测定广西牛大力药材中芒柄花素和高丽槐素的含量  

Determination of Maackiain and Formononetin in Root of Millettia Speciosa by HPLC

作　　者：陈勇[1] 谢臻[1] 巫繁菁[1] 韦玉燕[1] 卢森华[1] 曾海生[1] 

机构地区：[1]广西中医药大学药学院,南宁530001

出　　处：《世界科学技术-中医药现代化》2013年第2期260-263,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：广西壮族自治区中医药管理局2011年度中医药科技专项(GZKZ1113):牛大力药材的质量分析研究;负责人:陈勇;广西自然科学基金创新研究团队项目(2011GXNSFF018006):中药新药基础研究;负责人:朱华

摘　　要：目的：建立牛大力药材中芒柄花素和高丽槐素的含量测定方法。方法：采用HPLC测定药材中芒柄花素和高丽槐素含量，色谱柱C18。（4．6mm×250mm，5μm），流动相乙腈-0．1％冰醋酸溶液（38：62），检测波长248，310nm，流速1．0mL·min^-1，柱温35℃。结果：芒柄花素进样量在0．0023。0．0600μg与峰面积值呈现良好的线性关系（r=0．9999），平均回收率100．0％，RSD为2．0％（n=6）；高丽槐素进样量在0．0391～1．0156μg与峰面积值呈现良好的线性关系（r=0．9999），平均回收率为100．6％，RSD为0．7％（n=6）。结论：该方法可用于牛大力药材中芒柄花素和高丽槐素的含量测定。This study was aimed to establish a HPLC in root of MiUettia speciosa Champ. Diamonsil Cls（2） method for the determination of Maackiain and formononetin column was used as a chromatographic column. The mobile phase was acetonitrile-0.1% acetic acid solution （38：62）. The flow rate was 1.0 mL.min^-1 and the detection wavelengths were set at 248 and 310 nm. The column temperature was maintained at 35℃ with injection volume of 20 μL. The calibration curve of formononetin was in good linearity over the range of 0.002 3-0.060 01μg （r = 0.999 9）. The average recovery was 100.0% with RSD of 2.00% （n = 6）. And the calibration curve of Maackiain was in good linearity over the range of 0.039 1-1.015 6 Ixg （r = 0.999 9）. The average recovery was 100.6% with RSD of 0.70% （n = 6）. It was concluded that this method is suitable for the determination of Maackiain and formononetin in the root of M. speciosa.

关 键 词：牛大力 芒柄花素 高丽槐素 HPLC 

分 类 号：R284[医药卫生—中药学]