小剂量牛初乳短链胰岛素样生长因子-Ⅰ可改善糖尿病大鼠周围神经病变  

Bovine colostrum truncated insulin like growth factor-Ⅰ ameliorates peripheral neuropathy in diabetic rats

作　　者：许光武[1] 俞茂华[1] 叶红英[1] 颜贻谦[2] 

机构地区：[1]复旦大学医学院附属华山医院内分泌科,上海200040 [2]中科院上海生理研究所

出　　处：《中华内分泌代谢杂志》2000年第4期231-234,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的　研究小剂量牛初乳短链胰岛素样生长因子 Ⅰ (Bc tIGF Ⅰ )、重组人IGF Ⅰ(rhIGF Ⅰ )对链脲佐菌素 (STZ)诱导的糖尿病大鼠周围神经病变的作用。方法　早期给STZ糖尿病大鼠腹腔注射Bc tIGF Ⅰ和rhIGF Ⅰ (均为 75 0ng·kg-1·d-1)。 11周后对各组大鼠进行神经电生理及病理形态分析。结果　Bc tIGF Ⅰ治疗后坐骨神经运动神经传导速度、动作电位及H反射潜伏期都明显改善 (P <0 .0 5 ) ,神经病理也明显改善。rhIGF Ⅰ的治疗对坐骨神经运动传导速度、波幅及H反射潜伏期没有影响 (P >0 .0 5 ) ,神经病理也没有明显改善。结论　小剂量Bc tIGF Ⅰ (75 0ng·kg-1·d-1)能改善糖尿病神经病变 ,而同等剂量的rhIGF Ⅰ无此作用。Objective To study the effects of small dose of bovine colostrum truncated insulin like growth factor Ⅰ (Bc tIGF Ⅰ) and recombinant human insulin like growth factor Ⅰ (rhIGF Ⅰ) on peripheral neuropathy in streptozotocin (STZ) induced diabetic rats.Methods STZ induced diabetes mellitus (DM) rats were allocated to three groups. One group received no treatment, another group intraperitoneal injection with Bc tIGF Ⅰ, the third group with rhIGF Ⅰ. And there was also a normal control group. The duration of treatment was 11 weeks. Blood glucose was measured at various times after treatment. Electrophysiological measurements and morphologic analyses of sciatic nerve were performed. Several related serum hormones (insulin, C peptide and IGF Ⅰ) were also assayed. Results Motor nerve conduction velocity (MNCV) of sciatic nerve and H reflex in Bc tIGF Ⅰ treated group was significantly improved in contrast to that in DM group (P<0.05). However, treatment with rhIGF Ⅰ brings no improvement (P>0.05). The ultrastructural appearance showed that treatment with Bc tIGF Ⅰ could alleviate diabetic neuropathy, but it did not happen in rhIGF Ⅰ group. Blood glucose concentration was lowered by Bc tIGF Ⅰ but not rhIGF Ⅰ therapy. Neither Bc tIGF Ⅰ nor rhIGF Ⅰ therapy affected serum levels of insulin and C peptide (P>0.05). Conclusion Bc tIGF Ⅰ but not rhIGF Ⅰ can improve diabetic peripheral neuropathy at a lower dosage.

关 键 词：糖尿病周围神经病变 BC-tIGF-I RHIGF-I STZ 

分 类 号：R587.2[医药卫生—内分泌] R745.02[医药卫生—内科学]