基因Nelin对人大隐静脉平滑肌细胞表型转化的影响  

Effect of Nelin gene on phenotypic transition of human great saphenous vein smooth muscle cell PEI

作　　者：裴长安[1] 秦士勇[1] 陈士辉[1] 张曙光[1] 

机构地区：[1]山东大学附属千佛山医院血管外科,济南250014

出　　处：《中华实验外科杂志》2013年第5期925-927,F0003,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：山东省自然科学基金自筹经费项目（ZR2009CL018）

摘　　要：目的观察Nelin对血管平滑肌细胞（VSMC）表型转化的影响。方法血清饥饿诱导VSMC由合成型向收缩型转变，应用逆转录一聚合酶链反应（RT—PCR）技术检测不同表型VSMC的NelinmRNA表达水平；转染Nelin．小干扰RNA（siRNA）沉默Nelin的表达，应用考马斯亮蓝染色法、乙酰胆碱（Ach）刺激法观察VSMC细胞骨架和收缩功能的变化。结果NelinmRNA在血清刺激组与血清饥饿24、48、72h组表达量分别为：0．1940±0．0971、0．7114±0．1265、0．7270±0．1026、0．7841±0．1240，差异有统计学意义（P〈0．05）。在血清饥饿培养过程中，VSMC细胞骨架由稀疏、模糊网状重构为均匀、致密束状，并重获收缩功能。抑制Nelin表达后，血清饥饿诱导的细胞骨架重构受阻，Ach诱发的细胞收缩消失。结论Nelin参与VSMC骨架重塑并调节其收缩功能，对VSMC表型转化的调节、收缩表型的维持具有重要作用。Objective To investigate the effect of Nelin on phenotypic transition of vascular smooth muscle cells （VSMC）. Methods During reversion of synthetic VSMC to contractile phenotype induced by serum starvation, the expression of Nelin mRNA in different phenotypes was detected by reverse transcriptase-polymerase chain reaction （RT-PCR）. VSMC was transfected by Nelin-small interfering RNA （siRNA） to knock-down the expression of Nelin, and then observe the effects on cytoskeleton and contractibility by respectively coomassie brilliant blue dyeing and acetylcholine （ACh）-induction. Results The expression level of Nelin mRNA in VSMC Which were stimulated by serum and serum starvation for 24, 48, 72 h was 0. 1940 ± 0. 0971,0. 7114 ± 0. 1265,0. 7270 ± 0. 1026, 0. 7841 ± 0. 1240 respectively （P 〈 0. 05 ）. The cytoskeleton was remodulated from discrete and fuzzy network to well arranged and dense bundles after serum starvation. At the same time, ACh-induced contractility was observed. After Nelin expression was inhibited, reorganization of cytoskeleton by serum starvation was blocked, and Ach-induced contraction was absent. Conclusion Nelin was involved in VSMC cytoskeleton reorganization and played an important role for VSMC remaining contractile phenotype and phenotypic transition.

关 键 词：Nelin 血管平滑肌细胞 表型转化 细胞骨架 收缩 

分 类 号：R54[医药卫生—心血管疾病]