蚕丝-聚乳酸-聚羟基乙酸纤维混合编织绳状支架的生物学性能  

Biological property of a rope-shaped scaffold braided by silk-poly （ L-lactic acid） -polyglycolic acid fiber

作　　者：张文元[1] 杨亚冬[1] 张科技[1] 李颖[1] 房国坚[1] 

机构地区：[1]浙江省医学科学院生物工程研究所,杭州310013

出　　处：《中华实验外科杂志》2013年第5期931-933,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（81071467）;浙江省医学重点学科群项目生物医药重点学科群（XKQ4310-001）;浙江省医药卫生科技计划项目（2010KYB001）

摘　　要：目的通过对蚕丝丝素-聚乳酸（PLA）-聚羟基乙酸（PGA）纤维混合编织绳状支架的力学性能分析及生物学评价，探讨使用该支架构建组织工程韧带的可行性。方法将蚕丝-PLA—PGA纤维混合编织，捻拧成绳状支架，蚕丝丝素．PLA—PGA的质量比约为1：1：1，每个支架共含单丝7200根。使用电子万能试验机对支架材料进行力学性能测试，并进行如下生物学评价：细胞毒性实验、急性全身毒性试验、皮内刺激试验、热原试验、肌肉埋植实验，并进行分析、评价。结果蚕丝-PLA—PGA纤维混合绳状支架韧性强，为螺旋上升的绳索状，直径2．7mm，力学性能优良。支架无细胞毒性（细胞毒性分级0-1级），无急性全身毒性作用，无皮内刺激反应，不具热原反应，组织相容性良好。结论蚕丝-PLA—PGA编织绳状支架具有优良的力学性能及良好的生物相容性。Objective To evaluate mechanical property and biological property of a rope-shaped scaffold braided by silk-poly （L-lactic acid） （PLA）-polyglycolic acid （PGA） fiber, and investigate the feasibility of fabricating tissue-engineered ligament by using such scaffold. Methods Firstly, a hybrid rope-shaped scaffold containing monofilament 7200 was prepared by twisting a mixture of silkworm silk fibroin, PLA, and PGA fiber filament with a mass ratio of 1：1 ： 1. The biomechanical property of the abovementioned silk-PLA-PGA scaffold was measured by using the electric universal testing machine. Secondly, the biological property of the silk-PLA-PGA braided scaffold materials was assessed according to the relevant experiments, including cytotoxicity test, acute systematic toxicity test, intracutaneous irritation test, pyrogen test, and muscle implant test. The experimental results were analyzed and assessed by criterion. Results The appearance of the scaffold braided by silk-PLA-PGA was a strong obdurability with a diamter of 2. 7 mm. The scaffold had excellent mechanical property, good histoeompatibility, no cytotoxicity, no acute systematic toxicity, no skin stimulation, and no pyrogen reaction. Conclusion The scaffold has good biomechanical property and bioeompatibility.

关 键 词：蚕丝 聚乳酸 聚羟基乙酸 支架 力学性能 生物学性能 

分 类 号：R318.08[医药卫生—生物医学工程]