STR-PCR方法检测贵州小型香猪遗传变异性的研究  

Monitoring the genetic variation of Guizhou miniature pigs with STR-PCR

作　　者：黄中波[1] 牛荣[1] 魏泓[1] 甘世祥[2] 

机构地区：[1]第三军医大学实验动物中心,重庆400038 [2]贵阳中医学院,贵阳550002

出　　处：《第三军医大学学报》2000年第7期642-644,共3页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划项目!(G2 0 0 0 .0 1 61 0 6);国家"九五"重点科技攻关项目!(96A 2 3 0 6 0 1);国家自然科学基金

摘　　要：目的　准确分析贵州小型香猪种群遗传变异及近交程度。方法　采用 10对短串联重复序列(STR)引物 ,对贵州小型香猪个体进行PCR检测。结果　在检测到的 88个有效等位基因型中 ,纯合基因型5 0个 ,占 5 5 .7%。个体平均杂合率为 0 .40 81,位点平均杂合率为 0 .5 5 6 2。结论　贵州小型香猪具有一定遗传稳定性 ,符合封闭群动物遗传学特征。Objective To study the genetic variation and inbreeding of Guizhou miniature pigs. Methods DNA of ten Guizhou miniature pigs extracted to observe its genotype by STR PCR with 10 pairs of short tandem repeat (STR) as the primers. Results Fifty homozygostic genes (55.7%) were found in all 88 effective allele types. Average heterozygostic rate of individuals was 0.4081, and that of loci was 0.5562. Conclusion Guizhou miniature pigs are genetically stabe and satisfied the genetic standard of closed colony animal.

关 键 词：贵州小型香猪 遗传变异性 STR-PCR 

分 类 号：R346.5[医药卫生—基础医学]