高同型半胱氨酸血症对小鼠金属硫蛋白合成的影响  

Homocysteine induces metallothionein formation in mice

作　　者：李淑莲[1] 李载权[2] 张英[2] 薛琳[2] 许松[2] 王晓红[2] 唐朝枢[2] 

机构地区：[1]开封医学专科学校病理生理教研室,河南开封475001 [2]北京医科大学第一医院中心实验室,北京100034

出　　处：《中国病理生理杂志》2000年第8期678-680,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助! (No .39730 2 2 0 )

摘　　要：目的 :观察高同型半胱氨酸 (Hcy)血症时小鼠组织金属硫蛋白 (MT)含量的变化。方法 :小鼠腹腔注射Hcy复制高Hcy血症模型。观察肝、心和肾组织的MT和丙二醛 (MDA)水平。结果 :肝、心和肾组织在Hcy组MT水平较对照组分别高 2 10 % (P <0 0 1)、133 % ((P <0 0 5 )和 6 0 % (P <0 0 1)。而丙二醛水平则分别高6 %、5 2 % (P <0 0 5 )和 6 9% (P <0 0 5 )。注射Hcy同时用超氧化物歧化酶 (SOD)处理的动物 ,肝、心和肾组织MT含量较Hcy组分别低 2 2 % (P <0 0 5 )、33 % (P <0 0 5 )和 13% (P >0 0 5 )。丙二醛含量则分别降低 2 4% (P <0 0 5 )、2 1% (P <0 0 5 )和 48% (P <0 0 1)。组织MT水平与MDA含量呈显著正相关。结论AIM: To observe the changes of metallothionein (MT) in various tissues of mice during hyperhomocysteinemia. METHODS: Intraperitoneal injection of homocysteine into mice induced hyperhomocysteinemia. The contents of tissue MT and malondialdehyde (MDA) in liver, heart and kidney were determined. RESULTS: Compared with control group, tissue MT levels in Hcy-group animals were increased by 210% ( P <0.01) for liver, by 133% ( P <0.05) for heart and by 60% ( P <0.01) for kidney. Tissue MDA contents were increased by 6%, 52% ( P <0.05) and 69% ( P <0.05), respectively. However, in treated animals with Hcy plus superoxide dismutase (SOD), the tissue MT contents in liver, heart and kidney were decreased by 22% ( P <0.05),33% ( P <0.05) and 13% ( P >0.05),respectively, compared with Hcy alone group. Tissue MDA contents were decreased by 24% ( P <0.05), 21% ( P <0.05) and 48% ( P <0.01). There was a positive correlation between tissue MT content and MDA level. CONCLUSION: Hcy could induce tissue MT formation through oxidative stress.

关 键 词：金属硫蛋白 应激 高同型半胱氨酸血症 小鼠 

分 类 号：R589.302[医药卫生—内分泌]