RP-HPLC法测定二十五味珍珠丸中木香烃内酯和去氢木香内酯的含量  

Content Determination of Costunolide and Dehydrocostus Lactone in Tibetan Medicine Ershiwuwei Zhenzhu Pill by RP-HPLC

作　　者：刘鸿雁 姜专基[2] 陈德道 张樱山 陈丽娟 陈根平 

机构地区：[1]甘肃奇正藏药有限公司药品研发部,兰州730000 [2]甘肃省肿瘤医院乳腺科,兰州730050

出　　处：《中国药房》2013年第20期1904-1906,共3页China Pharmacy

基　　金：国家科技支撑计划课题(No.2007BAI311304)

摘　　要：目的:建立测定藏成药二十五味珍珠丸中木香烃内酯和去氢木香内酯含量的方法。方法:采用反相高效液相色谱法。色谱柱为Kromasil C18柱,流动相为甲醇-水(65:35,V/V),流速为1.0ml/min,检测波长为225nm,柱温为30℃,进样量为10μl。结果:木香烃内酯、去氢木香内酯检测质量浓度分别在12～120、17～170μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.9997和0.9996);平均加样回收率分别为100.90%和100.35%,RSD分别为1.23%和0.95%。结论:该方法专属性强、灵敏度高、重复性好,适用于二十五味珍珠丸的质量控制。OBJECTIVE：To develop a method for the content determination of costunolide and dehydrocostus lactone in Tibetan medicine Ershiwuwei zhenzhu pill.METHODS：RP-HPLC method was used.The separation was performed on Kromasil C18 column with methanol-water（65：35,V/V） as mobile phase at the flow rate of 1.0 ml/min.The detection wavelength was set at 225 nm,and column temperature was 30 ℃.The injection volume was 10 μl.RESULTS：The linear ranges were 12-120 μg/ml for costunolide（r=0.999 7） and 17-170 μg/ml for dehydrocostus lactone（r=0.999 6）.The average recoveries were 100.90%（RSD=1.23%） and 100.35%（RSD=0.95%）,respectively.CONCLUSIONS：The method has good specificity,high sensitivity and good repeatability.It can be used for the content determination of costunolide and dehydrocostus lactone in Tibetan medicine Ershiwuwei zhenzhu pill.

关 键 词：二十五味珍珠丸 木香烃内酯 去氢木香内酯 反相高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]