洛沙坦对大鼠心肌梗死时心肌细胞的作用  

Effect of losartan on myocytes in myocardium-infarcted rats

作　　者：申景平[1] 李瑞峰[1] 雷立权[2] 高广道[2] 李炯 卢兴[2] 

机构地区：[1]山东医科大学病理生理学教研室,济南250012 [2]西安医科大学病理生理学教研室,西安710000

出　　处：《基础医学与临床》2000年第4期42-44,47,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金!( No.39270315);"八五"攻关资助!( No.85-195-03-02)

摘　　要：应用Ｌａｎｇｅｎｄｏｒｆｆ灌流方法，分离培养成年大鼠心肌细胞（ ＭＣ），并借助同位素标记的底物掺入法，观察在不同处理因素下，洛沙坦（Ｌｏｓ）对大鼠梗塞ＭＣ的ＲＮＡ（１４Ｃ－ＵＲ）的蛋白质（３Ｈ－Ｌｅｕ）合成率的影响。结果发现，在相同浓度的Ａｎｇ　（１０－７ｍｏｌ／Ｌ）的作用下，心肌梗塞对照组（ＣＭＩ）的ＭＣ，其１４Ｃ－ＵＲ和３Ｈ－Ｌｅｕ掺入率均显著高于假手术（ＳＯ）组（Ｐ＜０．０５）：而两干预组（ＭＬ＋Ｌｏｓ组和ＭＩ＋Ｃａｐ组）的ＭＣ，其ＲＮ Ａ和蛋白质合成速率与 ＳＯ组无差异。 Ａｎｇ 　的上述作用，可分别被 ＡＴ１受体阻滞剂 Ｌｏｓ、 Ａｕｇ 　 拮抗剂（１．８Ａｎｇ　）和血管紧张素抗肽（ Ａｎｇ－ＡＰ）充全阻断。提示，洛沙坦通过阻断 Ａｎｇ 　对非梗塞区 ＭＣ的作用，抑制ＭＣ的 ＲＮＡ、蛋白质合成；长期应用（ ６周） Ｌｏｓ可阻抑 ＭＩ后心肌细胞肥大和降低其对 Ａｎｇ 　的反应性。With the Langendorff infusion and the isotope-labeled substrate uptake, we investigated the mechanism of losartan on the isolated myocytes from myocardium-infarcted rats. This study showed that the synthesis rates of RNA (14C-UR) and protein (3H-Leu) in MC from control MI group are significantly higher than those from the shamoperated (SO) one treated with the same concentration of Aug , respectively. On the other hand, the uptake rates of 3H-Leu, 14C-UR of MC from MI group treated with losartan or captopril are not significantly different from those of the SO one, respectively. These effects of Aug n on MC are abolished completely by angiotensin receptor antagonist-losartan, angiotensin antagonists-1.8 Aug and angiotensin antipeptide. The findings suggest that losartan could prevent the syntheses of RNA and protein in the MC treated with Aug ; Losartan could reduced the myocardial hypertrophy and the responsiveness of Aug on the MC from uninfarcted area.

关 键 词：心肌梗死 血管紧张素Ⅱ 心肌细胞 洛沙坦 

分 类 号：R542.22[医药卫生—心血管疾病]