姜黄素对人宫颈癌细胞株Hela细胞增殖的作用的研究以及p53表达的影响  

Effects of Curcumin on Cell Apoptosis and Expression of p53 of Human Cervical Carcinoma cell Line HeLa

作　　者：姜智慧[1] 陆晓媛[2] 周健 

机构地区：[1]徐州医学院研究生院,江苏徐州221000 [2]徐州医学院附属医院 [3]江苏省徐州妇幼保健院

出　　处：《中国医学创新》2013年第12期9-12,共4页Medical Innovation of China

基　　金：江苏省卫生厅康莱特肿瘤基金(编号:P200943)

摘　　要：目的:主要探讨姜黄素对人类宫颈癌Hela细胞中增殖作用的影响,以及细胞p53蛋白表达的影响。方法:本文通过不同浓度姜黄素干预人宫颈癌Hela细胞,采用MTT法检测药物对Hela细胞的增殖抑制率;RT-PCR和Western blot检测细胞凋亡相关蛋白p53表达。结果:MTT发现姜黄素对人宫颈癌Hela细胞有一定的抑制作用,并呈时间-剂量依赖性;RT-PCR结果显示p53m RNA的表达增加;western boltting显示p53蛋白的表达增加。结论:姜黄素可一直Hela细胞增殖,其机制可能是通过增加p53基因的表达而实现。Objective： To observe the effect of cuicnmin on proliferation of human cervical cancer Hela cells and expression of p53 protein. Method： In this paper, Hela cells were treated with curcumin at various concentrations for various hours, Then determined for proliferation activity by MTT method, for transcription levels of β-catenin and p53 mRNAs by RT-PCR, and for expressions of β -catenin and p53 protein by Western blot. Result： Curcumin showed dose- and time-dependent inhibitory effect on the proliferation of Hela cells.Meanwhile, curcumin increased the mRNA transcription and protein expression levels of β -catenin and p53.Conclusion ： Curcumin inhibited the proliferation of Hela ceils by a possible mechanism of increased the express of p53.

关 键 词：姜黄素 宫颈癌 P53MRNA P53蛋白 

分 类 号：R285.5[医药卫生—中药学]