Box-Behnken效应面法优化陈皮中橙皮苷及川陈皮素提取工艺  

Optimization of Extraction Technology of Hesperidin and Nobiletin from Citrus reticulata by Box-Behnken Design-Response Surface Methodology

作　　者：韦正[1] 杨丽[1] 李旻[1] 彭月[1] 银玲[1] 刘荣[1] 樊丹青[1] 刘友平[1] 

机构地区：[1]成都中医药大学药学院中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中国实验方剂学杂志》2013年第10期17-20,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072991);四川省教育厅自然科学重点项目(09ZA033)

摘　　要：目的:通过Box-Behnken效应面法优化陈皮中橙皮苷及川陈皮素的提取工艺。方法:采用HPLC测定橙皮苷及川陈皮素含量,以橙皮苷及川陈皮素提取率的总评"归一值"为指标,通过Box-Behnken试验考察提取温度、液料比、提取时间对提取工艺的影响,对结果进行二项式方程拟合,利用效应面法优化提取工艺并进行预测分析。结果:最佳提取工艺为温度77℃,液料比19∶1,提取1.6 h,乙醇体积分数70%;预测值与验证值偏差较小(<5%)。结论:优选的提取工艺稳定可行,实际值与预测值吻合度高,预测性良好。Objective： To optimize extraction process of hesperidin and nobiletin from Citrus reticulata by Box-Behnken design-response surface method. Method： The content of hesperidin and nobiletin was determined by HPLC.Independent variables were extraction temperature,liquid-solid ratio and extraction time,dependent variable was the overall desirability of extraction rate of hesperidin and nobiletin.No-linear mathematic models was used to estimate relationship between independent and dependent variables,response surface methodology was used to optimize extraction process,prediction was carried out by comparing the observed and predicted values. Result： Optimum extraction technology was as following：reflux extracted 1.6 h with 19 times the amount of 70% ethanol at 77 ℃.Deviation between the observed and predicted values was small（〈5%）. Conclusion： This optimized extraction technology was stable and feasible with high predictability.

关 键 词：陈皮 橙皮苷 川陈皮素 星点设计-效应面法 提取工艺 

分 类 号：R283.6[医药卫生—中药学]