Box-Behnken效应面法优化地乌总皂苷提取工艺  

Optimization of Extraction Technology for Total Saponins from Anemone flaccida by Box-Behnken Response Surface Methodology

作　　者：余子川[1] 余捷婧[1] 韩建伟[1] 

机构地区：[1]湖北中医药大学,武汉430061

出　　处：《中国实验方剂学杂志》2013年第10期50-53,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖北省科技厅自然科学研究计划项目(981P1420)

摘　　要：目的:优选地乌总皂苷的提取工艺条件。方法:以总皂苷提取率为指标,通过单因素试验和Box-Behnken效应面法考察加水量、提取时间、提取次数、浸泡时间等因素对提取工艺的影响。结果:最佳提取工艺为加8.8倍量水浸泡90 min,煎煮提取2次,每次2.5 h。地乌总皂苷提取率11.23%。结论:优选的提取工艺稳定可行,数学模型的预测值与试验观察值相符。Objective： To optimize extraction technology of total saponins from Anemone flaccida. Method： With extraction rate of total saponins as dependent variable,soaking time,the amount of water,extraction time as independent variables,effects of influencing factors on extraction technology was investigated by single factor test and Box-Behnken response surface methodology. Result： Optimum process parameters were obtained as followings：soaked 90 min with 8.8 times the amount of water,boiled 2 times with 2.5 hours per time.Extraction rate of total saponins was 11.23%. Conclusion： This optimized technology was stable and feasible,the predictive value of mathematical model and the experimental observation value was fitted.

关 键 词：地乌 总皂苷 提取工艺 BOX-BEHNKEN效应面法 

分 类 号：R283.6[医药卫生—中药学]