黄丝郁金和绿丝郁金中姜黄素类成分含量比较  

Quantitative Analysis of Curcuminoids in Huangsi Yujin and Lvsi Yujin Derived from Cucurmae Radix

作　　者：李锐[1] 和心依[2] 肖燕[1] 

机构地区：[1]西华大学生物工程学院,成都610039 [2]四川大学,成都610015

出　　处：《中国实验方剂学杂志》2013年第10期88-91,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国博士后科学基金项目(2012M511935);四川省教育厅重点项目(11ZA005);西华大学重点科研项目(E112053)

摘　　要：目的:采用HPLC高效液相色谱法对黄丝郁金和绿丝郁金中姜黄素、单脱甲氧基姜黄素、双脱甲氧基姜黄素的含量进行比较研究。方法:分别搜集10个批次的药材样品,以甲醇超声提取60 min,以YMC-Pack-ODS-A C18色谱柱(4.6 mm×250 mm,5μm)分离,流动相乙腈-0.1%甲酸溶液(45∶55),流速1.0 mL.min-1,检测波长425 nm,进行外标法定量分析。结果:姜黄素在3.516～450 mg.L-1呈良好的线性关系(r=0.999 8),平均回收率101.36%,RSD 2.62%(n=5);脱甲氧基姜黄素在2.734～350 mg.L-1呈良好的线性关系(r=0.999 9),平均回收率102.19%,RSD 2.75%(n=5);双脱甲氧基姜黄素在2.422～310 mg.L-1呈良好的线性关系(r=0.999 8),平均回收率102.86%,RSD 3.06%(n=5)。结论:文章所建立的HPLC方法可用于有效控制中药郁金的药材质量;黄丝郁金中含有姜黄素类成分,而绿丝郁金中未检测到姜黄素类成分。Objective： To develop an HPLC method for quantitative analysis of curcuminoids in Huangsi Yujin and Lvsi Yujin derived from Cucurmae Radix. Method： Ten batches of samples were collected respectively, and were extracted with methanol in ultrasonic bath for 60 min, the separation was performed on a YMC ODS-A C18 reversed column （4.6 mm×250 mm, 5 μm）, the mobile phase consisted of acetonitrile and 0.1% formic acid in water （45：55）, the mobile phase flow rate was 1.0 mLmin-1 and the UV detector was monitored 425 nm for quantitative analysis. Result： The linear of curcumin, demethoxycurcumin, bisdemethoxycurcumin were within 3.516-450 mgL-1 （r=0.999 8）, 2.734-350 mgL-1（r=0.999 9） and 2.422-310 mgL-1（r=0.999 8） respectively. The average recoveries of curcumin, demethoxycurcumin, bisdemethoxycurcumin were 101.36% （RSD 2.62%, n=5）, 102.19% （RSD 2.75%, n=5） and 102.86% （RSD 3.06%, n=5） respectively. Conclusion： The validated HPLC method could be used as quality control method for Cucurmae Radix. Curcuminoids were only detected in Huangsi Yujin, while not detected in Lvsi Yujin.

关 键 词：黄丝郁金 绿丝郁金 姜黄素类成分 高效液相色谱法 含量比较 

分 类 号：R284.1[医药卫生—中药学]