色谱-原子荧光光谱联用技术测定北柴胡中砷的含量  

Determination of As in Bupleurum chinense by Chromatography-AFS

作　　者：崔兰冲[1] 沈晓君[1] 蔡广知[1] 贡济宇[1] 

机构地区：[1]长春中医药大学国家中医药管理局中药分析三级实验室,长春130117

出　　处：《中国实验方剂学杂志》2013年第10期112-114,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省教育厅"十一五"科学技术研究项目(吉教科合字[2008]第89号)

摘　　要：目的:建立色谱-原子荧光光谱联用技术分离测定不同形态砷的方法。方法:色谱-原子荧光光谱联用技术对砷进行测定。色谱及光谱条件如下,阴离子分离柱PRP-X100(4.1 mm×250 mm,10μm),流动相15 mmoL.L-1(NH4)2HPO4(pH6.0),流速1 mL.min-1,柱温25℃。As空心阴极灯,灯电流/辅阴极灯电流:100/46 mA。结果:砷含量在0.002～0.01μg线性关系良好,平均回收率为97.35%(n=6),RSD 1.5%。结论:方法灵敏,准确,重复性好,是分离测试不同形态砷的有效方法。Objective： To establish the method of chromatography-AFS for separating and determining As from different existing forms in Bupleurum chinense. Method： Chromatography-AFS was used to determine the content of As. The conditions of chromatography and spectrum were as follows： negative ion column of PRP-X100 （4.1 mm×250 mm, 10 μm） was used, mobile phase was the solution of 15 mmoLL-1 （NH4）2HPO4 （pH 6.0）. The flow rate was 1 mLmin-1; column temperature was set at 25 ℃; element lamp was hollow cathode lamp of As, lamp current/auxiliary cathode lamp current was 100/46 mA. Result： The standard curve was linear in the range of 0.002-0.01 μg. The average recovery was 97.35% with RSD 1.5% （n=6）. Conclusion： The method is sensitive, accurate and reproducible. It can be used to determine the content of As in different existing forms.

关 键 词：北柴胡 色谱-原子荧光光谱联用 砷 含量测定 

分 类 号：R284.1[医药卫生—中药学]